C5467

EX-CELL^® ACF CHO Medium

Animal-component free, with HEPES, without L-glutamine, liquid, sterile-filtered, suitable for cell culture

• Synonym(s): CHO Medium
• MDL number: MFCD01634638
• UNSPSC Code: 12352207
• NACRES: NA.75

Properties

• Quality Level: 500
• description: for research or for further manufacturing use
• sterility: sterile-filtered
• form: liquid
• quality: Drug/Device Master File available
• technique(s): cell culture | mammalian: suitable
• components: HEPES: yes; NaHCO₃: yes; phenol red: no; L-glutamine: no
• shipped in: ambient
• storage temp.: 2-8°C

Description

Application

Animal component-free medium formulated to optimize cell growth and protein expression in Chinese hamster ovary (CHO) cells.

Features and Benefits

Developed to meet the needs of biotechnology and vaccine manufacturing, this medium supports rapid initial cell growth and high levels of protein expression in suspension cultures. It also supports high cell densities and maintenance of these densities of viable cells for extended periods resulting in increased productivity. Cell densities in excess of 8 × 10⁶ cells/ml have been achieved in batch culture systems. Increases in protein productivity up to 500% per cell have been observed.

Other Notes

Proprietary formulation containing inorganic salts, HEPES and sodium bicarbonate buffers, essential and non-essential amino acids, vitamins, recombinant human insulin, plant hydrolysates, other organic compounds, trace elements, and surfactants.
Does not contain antibiotics, antimycotics, L-glutamine, or transferrin. Contains no animal-derived proteins or other components.

Reconstitution

Aseptically add 20-40 ml of 200 mM L-glutamine solution per liter of medium prior to use.

Legal Information

EX-CELL is a registered trademark of Merck KGaA, Darmstadt, Germany

Safety Information

• Storage Class Code: 12 - Non Combustible Liquids
• WGK: nwg
• Flash Point(F): Not applicable
• Flash Point(C): Not applicable