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A3525

Sigma-Aldrich

Anti-Human IgE (ε-chain specific)−Alkaline Phosphatase antibody produced in goat

affinity isolated antibody, buffered aqueous glycerol solution

Synonym(s):

Goat Anti-Human IgE (ε-chain specific)−AP

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About This Item

MDL number:
MFCD00162428
UNSPSC Code:
12352203
NACRES:
NA.46

biological source

goat

Quality Level

200

conjugate

alkaline phosphatase conjugate

antibody form

affinity isolated antibody

antibody product type

secondary antibodies

clone

polyclonal

form

buffered aqueous glycerol solution

species reactivity

human

technique(s)

direct ELISA: 1:7,000-1:21,000

shipped in

wet ice

storage temp.

2-8°C

target post-translational modification

unmodified

Related Categories

General description

Affinity isolated antibody is obtained from anti-human IgE antiserum by immunospecific purification which removes essentially all goat serum proteins, including immunoglobulins, which do not specifically bind to the ε-chain of human IgE. Anti-Human IgE is conjugated to alkaline phosphatase by protein cross linking with 0.2% glutaraldehyde. IgE is present in low levels compared to other immunoglobulin isotypes. It exists in two forms, one being a secreted by plasma cell and the other form is associated with the B cell receptor.
IgEs are glycoprotein antibodies that regulate immunological responses to allergies and infections. These immunoglobulins have been implicated in mediating Type I hypersensitivity reactions. Elevated IgE levels have been associated with hyper IgE syndrome and allergic asthma . Anti-Human IgE (epsilon-chain specific)-Alkaline Phosphatase antibody is specific for human IgE when tested against human IgA, IgG, IgM, Bence Jones κ and λ myeloma proteins.

Immunogen

Purified human IgE

Application

Anti-Human IgE (ε-chain specific)−Alkaline Phosphatase antibody produced in goat has been used in dot blot.
Anti-Human IgE (epsilon-chain specific)-Alkaline Phosphatase antibody is suitable for use in immunoblotting and ELISA (at 1:500 dilutions using 100μ/well).
IgE secreted from transfected murin myeloma cells was detected by ELISA using the alkaline phosphatase-conjugated goat anti-human IgE (epsilon specific) antibody.

Biochem/physiol Actions

IgE bound to B cell receptor mediates antigen uptake and regulates differentiation and proliferation of B cells.

Physical form

Solution in 0.05 M Tris, pH 8.0, containing 1% bovine serum albumin, 1 mM MgCl2, 15 mM sodium azide and 50% glycerol

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

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Storage Class Code

10 - Combustible liquids

WGK

WGK 2

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

Regulatory Listings

Regulatory Listings are mainly provided for chemical products. Only limited information can be provided here for non-chemical products. No entry means none of the components are listed. It is the user’s obligation to ensure the safe and legal use of the product.

EU REACH Annex XVII (Restriction List)

CAS No.

Certificates of Analysis (COA)

Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.

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James A Gregory et al.
Plant biotechnology journal, 14(7), 1541-1550 (2016-01-24)
Peanut allergy is an IgE-mediated adverse reaction to a subset of proteins found in peanuts. Immunotherapy aims to desensitize allergic patients through repeated and escalating exposures for several months to years using extracts or flours. The complex mix of proteins
C D Lindstrøm et al.
Scandinavian journal of immunology, 44(4), 335-344 (1996-10-01)
Allergy to fish is common in Northern Europe. Variable reactions to different fish species are usually experienced among fish allergic patients. The allergens of cod fish and particularly the major allergen parvalbumin beta (Gadus callarias) have been extensively studied in
Yanfang Rui et al.
The Journal of neuroscience : the official journal of the Society for Neuroscience, 26(41), 10480-10487 (2006-10-13)
Defects in axonal transport are often associated with a wide variety of neurological diseases including Alzheimer's disease (AD). Beta-amyloid (Abeta) is a major component of neuritic plaques associated with pathological conditions of AD brains. Here, we report that a brief
Ping Zhong et al.
The Journal of biological chemistry, 278(29), 26888-26896 (2003-05-15)
It has long been recognized that muscarinic acetylcholine receptors (mAChRs) are crucial for the control of cognitive processes, and drugs that activate mAChRs are helpful in ameliorating cognitive deficits of Alzheimer's disease (AD). On the other hand, GABAergic transmission in
Serum proteins and fractions, HDL-cholesterol and total IgG and IgE levels in cases of acute and chronic paracoccidioidomycosis.
Marquez, Ade, S., et al.
Rev. Biochem., 42(3), 245-249 (2009)
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