93145
1,3,5-Triphenyltetrazolium formazan
≥90% (UV)
Synonym(s):
TTC formazan
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About This Item
Empirical Formula (Hill Notation):
C19H16N4
CAS Number:
Molecular Weight:
300.36
Beilstein:
753057
EC Number:
MDL number:
UNSPSC Code:
12352204
PubChem Substance ID:
NACRES:
NA.83
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Quality Level
Assay
≥90% (UV)
form
powder
solubility
dioxane: 50 mg/mL, clear
SMILES string
N(\N=C(/N=N/c1ccccc1)c2ccccc2)c3ccccc3
InChI
1S/C19H16N4/c1-4-10-16(11-5-1)19(22-20-17-12-6-2-7-13-17)23-21-18-14-8-3-9-15-18/h1-15,20H/b22-19-,23-21+
InChI key
BEIHVSJTPTXQGB-YMGPPEANSA-N
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General description
1,3,5-Triphenyltetrazolium formazan is an insoluble compound produced upon the reduction of 2,3,5-triphenyl-2H-tetrazolium chloride (TTC) salt, which is an indicator of cell redox potential. This reaction is irreversible. TTC formazan is brightly colored and can be quantified using a spectrophotometer.
Application
1,3,5-Triphenyltetrazolium formazan has been used:
- for the quantification of 2,3,5-triphenyltetrazolium chloride (TTC) reduction activity in bacterial cells
- as a standard to study the optical density of formazan produced by TTC-treated milled seeds
- to stain heart tissue for infarct size assessment or undergo perfusion fixation to allow morphometric studies in addition to histology and immunohistochemistry
Other Notes
Review: Tetrazolium salts and formazans
Signal Word
Warning
Hazard Statements
Precautionary Statements
Hazard Classifications
Acute Tox. 4 Oral - Aquatic Acute 1
Storage Class Code
11 - Combustible Solids
WGK
WGK 3
Flash Point(F)
Not applicable
Flash Point(C)
Not applicable
Personal Protective Equipment
dust mask type N95 (US), Eyeshields, Gloves
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