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87917

Sigma-Aldrich

Tetramethylrhodamine ethyl ester perchlorate

suitable for fluorescence, ≥90% (HPCE)

Synonym(s):

TMRE, TMRE perchlorate

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About This Item

Empirical Formula (Hill Notation):
C26H27ClN2O7
CAS Number:
115532-52-0
Molecular Weight:
514.95
MDL number:
MFCD00467973
UNSPSC Code:
12352116
PubChem Substance ID:
329769616
NACRES:
NA.32

Quality Level

100

Assay

≥90% (HPCE)

form

solid

solubility

DMSO: soluble
alcohols: soluble

fluorescence

λex 540 nm; λem 595 nm in DMSO

suitability

suitable for fluorescence

storage temp.

2-8°C

SMILES string

[O-]Cl(=O)(=O)=O.CCOC(=O)c1ccccc1-c2c3ccc(cc3[o+]c4cc(ccc24)N(C)C)N(C)C

InChI

1S/C26H27N2O3.ClHO4/c1-6-30-26(29)20-10-8-7-9-19(20)25-21-13-11-17(27(2)3)15-23(21)31-24-16-18(28(4)5)12-14-22(24)25;2-1(3,4)5/h7-16H,6H2,1-5H3;(H,2,3,4,5)/q+1;/p-1

InChI key

NBAOBNBFGNQAEJ-UHFFFAOYSA-M

General description

Tetramethylrhodamine ethyl ester perchlorate (TMRE), also known as TMRE perchlorate, is a potential mitochondrial dye. TMRE staining process is reversible, and it doesn’t affect cell proliferation and viability.

The sorted cells containing TMRE perchlorate don’t interfere with the subsequent functional assays and hence are a preferred choice for the enrichment of functionally active, unbiased cell populations.

Application

Tetramethylrhodamine ethyl ester perchlorate (TMRE) is used to study the effects of high glucose in inducing mitochondrial morphology and metabolic changes.

TMRE perchlorate is also used to study Fullerene C 60 penetration into Leukemic Cells and the subsequent photoinduced Cytotoxic Effects.

Packaging

Bottomless glass bottle. Contents are inside inserted fused cone.

Other Notes

Potential-sensitive probe for measuring membrane potential changes in mitochondria

Pictograms

Exclamation mark
GHS07

Signal Word

Warning

Hazard Statements

H302 + H332,H315,H319

Hazard Classifications

Acute Tox. 4 Oral - Eye Irrit. 2 - Skin Irrit. 2

Storage Class Code

13 - Non Combustible Solids

WGK

WGK 3

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

Personal Protective Equipment

dust mask type N95 (US), Eyeshields, Gloves

Certificates of Analysis (COA)

Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.

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Garima Sharma et al.
Journal of biochemical and molecular toxicology, 33(8), e22337-e22337 (2019-04-09)
Elevated levels of saturated fatty acids show a strong cytotoxic effect in liver cells. Sirtuin 3 (SIRT3), a mitochondrially localized member of NAD+ -dependent deacetylase has been shown to protect hepatocytes against the oxidative stress. The role of SIRT3 on
Phong Lu et al.
Molecular pharmaceutics, 16(8), 3386-3398 (2019-06-27)
Clinical trials involving p53 gene therapy for ovarian cancer failed due to the dominant negative inhibition of wild-type p53 and multiple genetic aberrations in ovarian cancer. To overcome this problem, we have designed a more potent chimeric gene fusion, called
Irina V Sorokina et al.
Scientific reports, 7(1), 14571-14571 (2017-11-08)
Evading cell death is a major driving force for tumor progression that is one of the main problems in current cancer research. Mitotic catastrophe (MC) represents attractive platform compromising tumor resistance to current therapeutic modalities. MC appeared as onco-suppressive mechanism
Yun Shan Chen et al.
Cancer management and research, 11, 7353-7367 (2019-09-10)
The role of endothelin receptor type B (EDNRB) isoform 3 involved in Temozolomide (TMZ)-induced melanoma cell death has not yet been elucidated. The subcellular localization of EDNRB isoform 3 was determined by confocal and immunoblotting assays. Silencing EDNRB isoform 3
Hai-Bo Li et al.
DNA and cell biology, 35(11), 680-690 (2016-10-12)
Mitochondrial ribosomal proteins are important for mitochondrial-encoded protein synthesis and mitochondrial function. In addition to their roles in mitoribosome assembly, several mitochondrial ribosome proteins are also implicated in cellular processes like cell cycle regulation, apoptosis, and mitochondrial homeostasis regulation. Here
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