58672C
GS Supplement 50X
50 ×, liquid, sterile-filtered, suitable for cell culture
Pharma Manufacturing
Synonym(s):
GSEM Supplement, Glutamine synthetase expression medium supplement
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About This Item
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description
for research or for further manufacturing use
Quality Level
sterility
sterile-filtered
form
liquid
concentration
50 ×
technique(s)
cell culture | mammalian: suitable
shipped in
dry ice
storage temp.
−20°C
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General description
The GS System™ uses the Glutamine synthetase (GS) gene as a selectable marker for the expression of recombinant proteins in mammalian cells, especially NS0 and CHO cells. The system has been shown to have significant advantages with respect to speed and efficiency of cell line development and has resulted in high productivity for antibodies and other recombinant proteins.
Application
GS Supplement 50X has been used to as a supplement for culture media to grow GS-NS0 mouse myeloma cells.
The glutamine synthetase (GS) system is used to express recombinant proteins from mammalian cells. Glutamine-dependent cells transfected with a glutamine synthetase gene can be selectively grown in a suitable glutamine-deficient medium.
Packaging
Contains (mg/L): L-alanine 450.0, L-asparagine · H2O 4261.0, L-aspartic acid 650.0, L-glutamic acid 3750.0, L-proline 575.0, L-serine 500.0, adenosine 350.0, guanosine 350.0, cytidine 350.0, uridine 350.0, and thymidine 12.0
Quantity
One mL will supplement 50 mL of cell culture medium.
Storage and Stability
Store GS Supplement 50X protected from light at -10 to -40 °C.
Legal Information
GS System is a trademark of Lonza Group Ltd.
Storage Class Code
12 - Non Combustible Liquids
WGK
WGK 1
Flash Point(F)
Not applicable
Flash Point(C)
Not applicable
Personal Protective Equipment
dust mask type N95 (US), Eyeshields, Gloves
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Cell and Tissue Culture: Laboratory Procedures, 27D, 3-3 (1999)
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