07985

6-Aminofluorescein

BioReagent, suitable for fluorescence, ~95% (HPLC)

• Synonym(s): Fluoresceinamine isomer II
• Empirical Formula (Hill Notation): C₂₀H₁₃NO₅
• CAS Number: 51649-83-3
• Molecular Weight: 347.32
• Beilstein: 51708
• EC Number: 257-334-7
• MDL number: MFCD00005051
• UNSPSC Code: 12171500
• PubChem Substance ID: 329748535
• NACRES: NA.32

Properties

• product line: BioReagent
• Assay: ~95% (HPLC)
• form: powder
• impurities: ≤5% 5-aminofluorescein
• mp: 285 °C (dec.) (lit.)
• solubility: DMSO: soluble; acetone: soluble; methanol: soluble
• fluorescence: λ_ex 490 nm; λ_em 520 nm in 0.1 M Tris pH 9.0
• suitability: suitable for fluorescence
• SMILES string: Nc1ccc2C(=O)OC3(c4ccc(O)cc4Oc5cc(O)ccc35)c2c1
• InChI: 1S/C20H13NO5/c21-10-1-4-13-16(7-10)20(26-19(13)24)14-5-2-11(22)8-17(14)25-18-9-12(23)3-6-15(18)20/h1-9,22-23H,21H2
• InChI key: YOAWSYSKQHLFPM-UHFFFAOYSA-N

Description

Application

6-Aminofluorescein has been demonstrated to be useful fluorescent labeling reagent for fullerene-based liposome nanostructures termed ‘buckysomes′. 6-aminofluorescein dissolved in 0.1 N NaOH can be quantified from a maximum absorbance at 490 nm. Decarboxylated 6-aminofluorescein dissolved 6 N HCl (prepare by constant boiling at 110° C for 24 h) can be quantified from a maximum absorbance at 454 nm. Aldehyde groups are formed by oxidateive damage to proteins, which can be detected by conjugation to decarboxylated 6-aminofluorescein.

Other Notes

Note: Do not confuse with fluorescamine.

Safety Information

• Storage Class Code: 11 - Combustible Solids
• WGK: WGK 3
• Flash Point(F): Not applicable
• Flash Point(C): Not applicable
• Personal Protective Equipment: dust mask type N95 (US), Eyeshields, Gloves