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Y1500

Sigma-Aldrich

YPD Agar

Synonym(s):

Yeast Extract Peptone Dextrose Agar, YEPD Agar

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About This Item

UNSPSC Code:
50405901
eCl@ss:
42040102
NACRES:
NA.75

form

powder

Quality Level

composition

Agar, 15 g/L
Bacteriological peptone, 20 g/L
Glucose, 20 g/L
Yeast extract, 10 g/L

application(s)

food and beverages

suitability

nonselective for Candida spp.
nonselective for Pichia spp.
nonselective for Saccharomyces spp.
nonselective for Zygosaccharomyces spp.

General description

YPD agar is a complete yeast culture medium for most yeast species, especially for Saccharomyces cerevisiae. It is also known as YEPD and comprises of yeast extract, bacteriological peptone, glucose, and agar. The components of this media provide all the necessary nutrients for yeast growth. The essential vitamins, minerals and amino acids in the medium acts carbon and nitrogen source.

Application

YPD agar has been used as a culture media:
  • to cultivate S. cerevisiae strains to find a long-term preservation method for a deep space biosensor mission
  • for yeast strains in in vitro broth microdilution (BMD) based on minimum inhibitory concentration (MIC)
  • for Zymoseptori triciti strains for virulence studies

Preparation Note

1. Suspend 65 g in 1 L of distilled water.
2. Heat to boiling while stirring to dissolve all ingredients completely.
3. Autoclave for 15 minutes at 121°C.

Storage Class Code

11 - Combustible Solids

WGK

WGK 3

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

Personal Protective Equipment

dust mask type N95 (US), Eyeshields, Gloves

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Ingrid A Edwards et al.
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Ammonia nitrogen and natural organic matter (NOM) seriously degrade the quality of surface waters. In this study, the optimum preparation conditions of a yeast-chitosan bio-microcapsule of the Candida tropicalis strain, used to treat micro-polluted surface water, were investigated. Fourier transform
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Articles

Technical Article on yeast media. Yeasts are eukaryotic microorganisms whose genomes have been comprehensively studied and some have been sequenced.

Protocols

Yeasts are considered model systems for eukaryotic studies as they exhibit fast growth and have dispersed cells. Yeast cultures can be grown, maintained, and stored in liquid media or on agar plates using techniques similar to those for bacterial cultures.

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Technical Article on yeast media. Yeasts are eukaryotic microorganisms whose genomes have been comprehensively studied and some have been sequenced...

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