L5382

Light Green SF Yellowish

crystalline

• Synonym(s): Acid Green 5
• Linear Formula: C₃₇H₃₄N₂O₉S₃Na₂
• CAS Number: 5141-20-8
• Molecular Weight: 792.85
• Colour Index Number: 42095
• Beilstein: 5717828
• EC Number: 225-906-5
• MDL number: MFCD00012121
• UNSPSC Code: 12171500
• PubChem Substance ID: 24896400
• NACRES: NA.47

Properties

• product name: Light Green SF Yellowish, powder or crystals
• form: powder or crystals
• Quality Level: 100
• technique(s): titration: suitable
• color: dark red to purple
• mp: 288 °C (dec.) (lit.)
• solubility: water: 10 mg/mL, clear
• εmax: >600 at 631-634 nm in water
• application(s): diagnostic assay manufacturing; hematology; histology
• storage temp.: room temp
• SMILES string: [Na+].[Na+].CCN(Cc1cccc(c1)S([O-])(=O)=O)c2ccc(cc2)\C(=C3\C=CC(\C=C3)=[N+](/CC)Cc4cccc(c4)S([O-])(=O)=O)c5ccc(cc5)S([O-])(=O)=O
• InChI: 1S/C37H36N2O9S3.2Na/c1-3-38(25-27-7-5-9-35(23-27)50(43,44)45)32-17-11-29(12-18-32)37(31-15-21-34(22-16-31)49(40,41)42)30-13-19-33(20-14-30)39(4-2)26-28-8-6-10-36(24-28)51(46,47)48;;/h5-24H,3-4,25-26H2,1-2H3,(H2-,40,41,42,43,44,45,46,47,48);;/q;2*+1/p-2
• InChI key: DGOBMKYRQHEFGQ-UHFFFAOYSA-L

Description

General description

Light green SF yellowish (LGSFY) is a disodium salt of an acid triphenylmethane dye. It is also known as Light Green, Lissamine Green SF, Food Green 2, Green No. 205, Acid Green, Acid Brilliant Green 5, and Acid Brilliant Green SF. LGSFY is an important component of Papanicolaou stains in addition to eosin Y and bismark brown Y. Light green SF yellowish is a histological stain for collagen and a permitted colorant for foodstuffs and beverages.

Application

Light green SF is generally used as a cytoplasmic counterstain with nuclear stains like safranine and Feulgen stain. In histopatology, it is used for collagen fiber staining in Masson′s trichrome, Papanicolaou cytological polychrome stains, and Twort stain for microorganisms in tissue sections. It is a constituent of polychrome staining method used for differentiating secretory cells of the pituitary gland. Light green SF also stains the cellulose cell walls of plant cells.

Safety Information

• Storage Class Code: 11 - Combustible Solids
• WGK: WGK 2
• Flash Point(F): Not applicable
• Flash Point(C): Not applicable
• Personal Protective Equipment: dust mask type N95 (US), Eyeshields, Gloves