C1484

7-Diethylamino-3-(4-maleimidophenyl)-4-methylcoumarin

≥95% (HPLC), solid

• Synonym(s): 1H-Pyrrole-2,5-dione, 1-(4-(7-(diethylamino)-4-methyl-2-oxo-2H-1-benzopyran-3-yl)phenyl)-, CPM
• Empirical Formula (Hill Notation): C₂₄H₂₂N₂O₄
• CAS Number: 76877-33-3
• Molecular Weight: 402.44
• MDL number: MFCD00077334
• UNSPSC Code: 12171500
• PubChem Substance ID: 24892413
• NACRES: NA.47

Properties

• Quality Level: 100
• Assay: ≥95% (HPLC)
• form: solid
• color: yellow
• solubility: DMSO: soluble; methanol: soluble
• application(s): diagnostic assay manufacturing; hematology; histology
• storage temp.: −20°C
• SMILES string: CCN(CC)c1ccc2C(C)=C(C(=O)Oc2c1)c3ccc(cc3)N4C(=O)C=CC4=O
• InChI: 1S/C24H22N2O4/c1-4-25(5-2)18-10-11-19-15(3)23(24(29)30-20(19)14-18)16-6-8-17(9-7-16)26-21(27)12-13-22(26)28/h6-14H,4-5H2,1-3H3
• InChI key: YGIABALXNBVHBX-UHFFFAOYSA-N

Description

General description

7-Diethylamino-3-(4-maleimidophenyl)-4-methylcoumarin (CPM), is a highly fluorescent, sulfhydryl (-SH) group containing coumarin derivative. It has excitation an emission wavelengths of 355 and 460 nm, respectively.

Application

7-Diethylamino-3-(4-maleimidophenyl)-4-methylcoumarin has been used:

• as a nontoxic substitute for conjugation with antibody(93)
• to aid fluorescent detection of Coenzyme A (CoA-SH) in human N-myristoyltransferases assay (94)
• in thermostability shift assay of recombinant protein(95)

Biochem/physiol Actions

Used to monitor release of thiols, quantitate thiol in microplate reactions, and to distinguish proliferating cancer cells by nucleolar protein staining.

Safety Information

• Storage Class Code: 11 - Combustible Solids
• WGK: WGK 3
• Flash Point(F): Not applicable
• Flash Point(C): Not applicable
• Personal Protective Equipment: dust mask type N95 (US), Eyeshields, Gloves