B3051

Brucella broth base

suitable for microbiology, NutriSelect^® Plus

• UNSPSC Code: 41171607
• NACRES: NA.85

Properties

• sterility: non-sterile
• Quality Level: 200
• form: powder
• shelf life: limited shelf life, expiry date on the label
• manufacturer/tradename: NutriSelect^® Plus
• technique(s): microbiological culture: suitable
• pH: 7.0±0.2 (25 °C)
• application(s): clinical testing; environmental; food and beverages; sample preparation; microbiology
• suitability: selective for Brucella spp.; selective for Campylobacter spp.

Description

Application

Recommended for enrichment, isolation and cultivation of Brucella species.

Components

Components (g/L):
Casein Enzymic Hydrolysate 10.00
Peptic Digest of Animal Tissue 10.00
Yeast Extract 2.00
Dextrose 1.00
Sodium Chloride 5.00
Sodium bisulphite 0.10

Preparation Note

Suspend 14.05 g of Brucella Broth Base in 500 ml of distilled water. Heat to boiling to dissolve the medium completely. Sterilize by autoclaving at 15 lbs. pressure (121°C) for 15 minutes. Cool to 45-50°C and aseptically add sterile 5% v/v heat inactivated (H1138) horse serum. Add polymyxin B (2,500 IU, Cat. No. 81334), bacitracin (12,500 IU, Cat. No. 11702), nystatin (50,000 IU, Cat. No. 74721), cycloheximide (50mg, Cat. No. 01810), nalidixic acid (2.5mg, Cat. No. 70162) and vancomycin (10mg, Cat. No. 94747) to make the medium selective. Mix well before pouring into sterile petri plates or tubes.
For isolation of the Campylobacter species add the rehydrated contents of one vial of Campylobacter Growth Supplement (Cat. No. C2847) and 5-7% defibrinated sheep blood to 500 ml of medium base. 500 ml of medium base. The Campylobacter Growth Supplement can also be made by mixing 125 mg sodium pyruvate (Cat. No. 15990), 125 mg sodium metabisulfite (Cat. No. 71928 )and 125 mg ferrous sulfate (Cat. No. 44970) in 2 ml of distilled water and filtering the solution through a sterile 0.4 mm filter to sterilize.

Footnote

We offer two media types: the superior granulated GranuCult^® and the cost-efficient powdered NutriSelect^® culture media, depending on your needs.
The designations basic, plus, or prime are added to indicate the quality control level, from basic quality control to standard QC plus to prime for full regulatory compliance.

Legal Information

GRANUCULT is a registered trademark of Merck KGaA, Darmstadt, Germany

NutriSelect is a registered trademark of Merck KGaA, Darmstadt, Germany

Safety Information

• Storage Class Code: 11 - Combustible Solids
• WGK: WGK 3
• Flash Point(F): Not applicable
• Flash Point(C): Not applicable
• Personal Protective Equipment: dust mask type N95 (US), Eyeshields, Gloves