16658

X-GalNAc

reagent for selection of recombinant bacterial clones

• Synonym(s): 5-Bromo-4-chloro-3-indolyl 2-acetamido-2-deoxy-β-D-galactopyranoside, 5-Bromo-4-chloro-3-indolyl N-acetyl-β-D-galactosaminide, X-Galactosaminide
• Empirical Formula (Hill Notation): C₁₆H₁₈BrClN₂O₆
• CAS Number: 129572-48-1
• Molecular Weight: 449.68
• MDL number: MFCD00083260
• UNSPSC Code: 12352200
• PubChem Substance ID: 329751315

Properties

• grade: for molecular biology
• sterility: non-sterile
• Assay: ≥98.0% (TLC)
• form: powder
• mp: ≥220 °C (lit.)
• solubility: DMF: soluble; DMSO: soluble
• suitability: suitable for β-galactosidase test
• storage temp.: −20°C
• SMILES string: CC(=O)N[C@@H]1[C@@H](O)[C@@H](O)[C@@H](CO)O[C@H]1Oc2c[nH]c3ccc(Br)c(Cl)c23
• InChI: 1S/C16H18BrClN2O6/c1-6(22)20-13-15(24)14(23)10(5-21)26-16(13)25-9-4-19-8-3-2-7(17)12(18)11(8)9/h2-4,10,13-16,19,21,23-24H,5H2,1H3,(H,20,22)/t10-,13-,14+,15-,16-/m1/s1
• InChI key: SUWPNTKTZYIFQT-LMXXTMHSSA-N

Description

General description

5-Bromo-4-chloro-3-indolyl N-acetyl-β-D-galactosaminide (X-GalNAc)is a chromogenic substrate for for N-acetyl-β-galactosidase, used to determine the presence or absence of a cloned DNA insert in bacteria growing on agar plates.

Application

Suitable for use in selection of recombinant bacterial colonies with the lac+ phenotype. Also suitable for the detection of Candida albicans.

Principle

When X-GalNAc is hydrolyzed by β-galactosidase, the resulting product will form a blue precipitate. Lac+ colonies grown in the presence of X-GalNAc turn blue in color, allowing for easy differentiation between lac+ and lac- colonies.