General description
Cytokines are immunomodulatory polypeptides that play key roles in both adaptive and innate immune responses.“Cytokine” is a general term used for a diverse group of soluble proteins and peptides which act as regulators under both normal and pathological conditions to modulate the functional activities of individual cells and tissues. These proteins also mediate direct interactions between cells and regulate processes taking place in the extracellular environment. Cytokines differ from hormones in that they act on a wider spectrum of target cells. The cytokine group of proteins includes lymphokines, interferons, colony stimulating factors and chemokines. Growth factors are involved in the stimulation of target cell survival, proliferation, differentiation with effects on angiogenesis, vasculogenesis, cell migration, apoptosis, wound healing and embryogenesis.Cytokine, chemokine and growth factor research plays a significant role in achieving a deeper understanding of the immune system and its multi-faceted response to most antigens, especially those responses that make up the inflammatory process, and disease states such as infectious disease, osteoarthritis, respiratory disease, IBD, sepsis, allergic reactions as well as neurologic, metabolic and cardiovascular disease and even cancer. MILLIPLEX® MAP offers the broadest selection of analytes across a wide range of disease states and species. Once the analytes of interest have been identified, you can rely on the quality that we build into each kit to produce results you can trust. In addition to the assay characteristics listed in the protocol, other performance criteria evaluated during the verification process include: cross-reactivity, dilution linearity, kit stability, and sample behavior (e.g. detectability and stability). Each MILLIPLEX® MAP panel and kit includes:Quality controls (QCs) to qualify assay performanceComparison of standard (calibrator) and QC lots to a reference lot to ensure lot-to-lot consistencyOptimized serum matrix to mimic native analyte environment in serum and plasma samplesDetection antibody cocktails designed to yield consistent analyte profiles within panelIn addition each panel and kit meets stringent manufacturing criteria to ensure batch-to-batch reproducibility. The MILLIPLEX® MAP Human Cytokine/Chemokine/Growth Factor Panel A thus enables you to focus on the therapeutic potential of cytokines and the modulation of cytokine expression. Coupled with the Luminex® xMAP platform in a magnetic bead format, you receive the advantage of ideal speed and sensitivity, allowing quantitative multiplex detection of dozens of analytes simultaneously, which can dramatically improve productivity.
Application
The Human Cytokine/Chemokine/Growth Factor Panel A bead-based multiplex panel, using the Luminex® xMAP technology, enables the simultaneous analysis of 38 multiple cytokine, chemokine, and growth factor biomarkers in human serum, plasma and cell culture samples.This is an overnight or two-hour incubation assay.This assay requires 25 μL of neat plasma or serum (1:100 dilution for RANTES) or 25 μL cell culture supernatant per well.This kit may be used for the analysis of a selected set of 38 analytes from the Human Cytokine/Chemokine/Growth Factor Panel A in human tissue/cell lysate and culture supernatant samples and serum or plasma samples.RANTES cannot be plexed with other cytokines in this panel due to a required 1:100 dilution of plasma/serum samples.This premixed panel comes with a RANTES bead, so please plan your samples accordingly.Analytes available:EGF;Eotaxin;G-CSF;GM-CSF;IFNα2;IFNγ;IL-1α;IL-1β;IL-1ra;IL-2;IL-3;IL-4;IL-5;IL-6;IL-7;IL-8;IL-10;IL-12 (p40);IL-12 (p70);IL-13;IL-15;IL-17A;IL-17E/IL-25;IL-17F;IL-18;IL-22;IP-10;MCP-1;M-CSF;MIG;MIP-1α;MIP-1β;PDGF-AA;PDGF-AB/BB;RANTES;TNFα;TNFβ;VEGF-A
Features and Benefits
Configuration: Premixed
Storage and Stability
Recommended storage for kit components is 2 - 8°C.
Legal Information
Luminex is a registered trademark of Luminex Corp
MILLIPLEX is a registered trademark of Merck KGaA, Darmstadt, Germany
xMAP is a registered trademark of Luminex Corp
Disclaimer
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
