FCMAB115F

Anti-TRA-1-60 Antibody, clone TRA-1-60, FITC conjugate

clone TRA-1-60, from mouse, FITC conjugate

• UNSPSC Code: 12352203
• eCl@ss: 32160702
• NACRES: NA.41

Properties

• biological source: mouse
• Quality Level: 100
• conjugate: FITC conjugate
• antibody form: purified antibody
• antibody product type: primary antibodies
• clone: TRA-1-60, monoclonal
• species reactivity (predicted by homology): human (based on 100% sequence homology)
• technique(s): flow cytometry: suitable; immunocytochemistry: suitable
• isotype: IgM
• shipped in: wet ice
• target post-translational modification: unmodified
• Gene Information: human ... PODXL(5420)

Description

General description

Human embryonal carcinoma (EC) cells are the stem cells of teratocarcinomas, and they are key components of germ cell tumors (GCTs). They express several high molecular weight glycoprotein antigens that are down-regulated upon differentiation. One of these antigens, defined by monoclonal antibody TRA-1-60, can be detected in the serum of GCT patients and provides a useful complement to the established serum markers human chorionic gonadotropin and α-fetoprotein, especially in those patients without elevated serum human chorionic gonadotropin or α-fetoprotein.

Specificity

This antibody reacts with TRA-1-60 antigen that is expressed upon the surface of human tetracarcinoma stem cells (EC), human embryonic germ cells (EG) and human embryonic stem cells (ES).
No immunoreactivity is seen with murine EC, EG, or ES cells. Both the TRA-1-60 and TRA-1-81 monoclonal antibodies (MAB4381) recognize antigens that are associated with a pericellular matrix proteoglycan. TRA-1-60 reacts with a sialidase-sensitive epitope whilst TRA-1-81 reacts with an unknown epitope of the same molecule

Immunogen

- Whole Cells corresponding to the of TRA-1-60.

Application

Detect TRA-1-60 using this Anti-TRA-1-60 Antibody, clone TRA-1-60, FITC conjugate validated for use in FC & IC.

Immunocytochemical staining of fixed H9 human ES cells incubated for two hours at 2-8C with 1:100 dilution of anti-TRA-1-60 FITC conjugated (Cat. No. FCMAB115F) monoclonal antibody. Pluripotent human ES cells exhibit strong immunoreactivity to this antibody.
Immunocytochemical staining of live H9 human ES cells incubated for 30 minutes at 37C with 1:100 dilution of anti-TRA-1-60 FITC conjugated (Cat. No. FCMAB115F) monoclonal antibody. Pluripotent human ES cells exhibit strong immunoreactivity to this antibody.
Flow Cytometry:
Antibody dilution for cellular staining:

• Prepare an antibody working solution by diluting 1:5 the primary antibody with PBS.
• Dispense the volume per test of working of solution according to the number of cells indicated in the table below.
• 5 L for Guava Flow Cytometer
• 10 L for other Flow Cytometry instruments

5 μl Working Solution: 5 x 10⁵ Cells / 95 μl PBS. 100 μl Total Reaction Volume
10 μl Working Solution: 1 x 10⁶ Cells / 90 μl PBS. 100 μl Total Reaction Volume

Research Category
Stem Cell Research

Research Sub Category
Pluripotent & Early Differentiation

Target description

~235/410 kDa

Physical form

Purified mouse monoclonal IgM conjugated to FITC in PBS with less than 0.09% sodium azide and 15 mg/mL BSA.

Storage and Stability

Maintain refrigerated at 2-8ºC protected from light in undiluted aliquots for up to 6 months from date of receipt.

Analysis Note

Control
Pluripotent human embryonic stem (ES) cells

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Safety Information

• Storage Class Code: 10 - Combustible liquids
• WGK: WGK 2
• Flash Point(F): Not applicable
• Flash Point(C): Not applicable