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Key Documents

ABE460

Sigma-Aldrich

Anti-dimethyl Histone H3 (Arg2), symmetric Antibody

serum, from rabbit

Synonym(s):

H3R2me2s, Histone H3/a, Histone H3/b, Histone H3/c, Histone H3/d, Histone H3/f, Histone H3/h, Histone H3/i, Histone H3/j, Histone H3/k, Histone H3/l

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About This Item

UNSPSC Code:
12352203
eCl@ss:
32160702
NACRES:
NA.41

biological source

rabbit

Quality Level

100

antibody form

serum

antibody product type

primary antibodies

clone

polyclonal

species reactivity

human

species reactivity (predicted by homology)

mouse (based on 100% sequence homology), rat (based on 100% sequence homology)

technique(s)

ChIP: suitable
dot blot: suitable
immunohistochemistry: suitable
western blot: suitable

NCBI accession no.

NP_003520

UniProt accession no.

P68431

shipped in

wet ice

target post-translational modification

dimethylation (Arg2)

Gene Information

human ... HIST1H3F(8968)

General description

Histones are highly conserved proteins that serve as the structural scaffold for the organization of nuclear DNA into chromatin. The four core histones, H2A, H2B, H3, and H4, assemble into an octamer (2 molecules of each). Subsequently, 146 base pairs of DNA are wrapped around the octamer, forming a nucleosome, the basic subunit of chromatin. Histones are modified post-translationally by the actions of enzymes in both the nucleus and cytoplasm. These modifications regulate DNA transcription, repair, recombination, and replication. The most commonly studied modifications are acetylation, phosphorylation, methylation, and ubiquitination. These modifications can alter local chromatin architecture, or recruit trans-acting factors that recognize specific histone modifications (the "histone code" hypothesis). The modifications occur predominantly on the N-terminal and C-terminal tails that extend beyond the nucleosome core particle.

Immunogen

Epitope: Methylated Arg2
Linear peptide corresponding to human Histone H3 symmetrically dimethylated at Arg2.

Application

Dot Blot (Specificity) Analysis: A 1:1,000 dilution from a representative lot detected Histone H3 (Arg2) in an Absurance Histone H3 Specificity Array (Cat. No. 16-667).

Immunohistochemistry Analysis: A representative lot from an independent laboratory detected Histone H3 (Arg2) in drosophila chromosomes (Migliori, V., et al. (2012). Nat Struct Mol Biol. 19(2):136-144.).

Chromatin Immunoprecipitation Analysis: A representative lot from an independent laboratory immunoprecipitated Histone H3 (Arg2) from P493-6 cell lysate (Migliori, V., et al. (2012). Nat Struct Mol Biol. 19(2):136-144.).
Research Category
Epigenetics & Nuclear Function
Research Sub Category
Histones
This Anti-dimethyl Histone H3 (Arg2) antibody, symmetric is validated for use in western blotting, dot blot, IHC & ChIP for the detection of dimethyl Histone H3 (Arg2).

Quality

Evaluated by Western Blotting in untreated and nocodazole treated HeLa cell lysate.

Western Blotting Analysis: A 1:1,000 dilution of this antibody detected dimethyl Histone H3 (Arg2) in 10 µg of nocodazole treated HeLa cell lysate and demonstrated a loss of signal in untreated HeLa cell lysate.

Target description

~17 kDa observed. Uncharacterized band(s) may be observed in some cell lysates.

Physical form

Rabbit polyclonal serum containing 0.05% sodium azide.
Unpurified

Storage and Stability

Stable for 1 year at -20°C from date of receipt.
Handling Recommendations: Upon receipt and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

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Storage Class Code

10 - Combustible liquids

WGK

WGK 1

Certificates of Analysis (COA)

Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.

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Valentina Migliori et al.
Nature structural & molecular biology, 19(2), 136-144 (2012-01-11)
The asymmetric dimethylation of histone H3 arginine 2 (H3R2me2a) acts as a repressive mark that antagonizes trimethylation of H3 lysine 4. Here we report that H3R2 is also symmetrically dimethylated (H3R2me2s) by PRMT5 and PRMT7 and present in euchromatic regions.
Yanbo Wang et al.
Scientific reports, 5, 11031-11031 (2015-06-16)
During germ cell development, epigenetic modifications undergo extensive remodeling. Abnormal epigenetic modifications usually result in germ cell loss and reproductive defect. Prmt5 (Protein arginine methyltransferase 5) encodes a protein arginine methyltransferase which has been demonstrated to play important roles in
Shivali Kapoor et al.
Proceedings of the National Academy of Sciences of the United States of America, 112(39), 12139-12144 (2015-09-16)
Point centromeres are specified by a short consensus sequence that seeds kinetochore formation, whereas regional centromeres lack a conserved sequence and instead are epigenetically inherited. Regional centromeres are generally flanked by heterochromatin that ensures high levels of cohesin and promotes

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