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70922

Millipore

BugBuster® HT Protein Extraction Reagent

Synonym(s):

Cell lysis reagent, Protein extraction buffer

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About This Item

UNSPSC Code:
41106511
NACRES:
NA.77

form

liquid

Quality Level

100

manufacturer/tradename

Novagen®

storage condition

OK to freeze
avoid repeated freeze/thaw cycles

shipped in

wet ice

storage temp.

2-8°C

Related Categories

General description

BugBuster HT Protein Extraction Reagent combines BugBuster Protein Extraction Reagent and Benzonase® Nuclease in one convenient reagent. BugBuster HT eliminates common bioprocessing problems resulting from traditional cell lysis procedures. Soluble proteins are gently extracted from E. coli without exposure to heat or oxidative damage and viscosity is eliminated by nucleic acid digestion in a single step. The resulting protein extract can easily be fractionated by conventional purification techniques. BugBuster HT is ideally suited for application in high throughput protein purifications.

Application

BugBuster® HT Protein Extraction Reagent has been used:
  • to lyse the Escherichia coli cell pellets following centrifugation for protein expression and immunoblotting
  • for protein extraction from bacterial cell pellet
  • used to lyse the E. coli cell pellets for the expression and purification of dMIC60 (mitofilin) protein

Features and Benefits

  • Fast protein extraction and nucleic acid degradation
  • Ideal for processing various samples of different volumes
  • Increased extraction is ensured by the addition of rLysozyme solution

Warning

Toxicity: Standard Handling (A)

Legal Information

BUGBUSTER is a registered trademark of Merck KGaA, Darmstadt, Germany
Benzonase is a registered trademark of Merck KGaA, Darmstadt, Germany
NOVAGEN is a registered trademark of Merck KGaA, Darmstadt, Germany

Storage Class Code

10 - Combustible liquids

WGK

WGK 2

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

Certificates of Analysis (COA)

Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.

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Chiara Pedicone et al.
iScience, 25(4), 104170-104170 (2022-04-26)
Here, we describe the use of artificial intelligence to identify novel agonists of the SH2-containing 5' inositol phosphatase 1 (SHIP1). One of the compounds, K306, represents the most potent agonist identified to date. We find that K306 exhibits selectivity for

Articles

Citation Spotlight: BugBuster® Protein Extraction Reagent for Efficient Protein Extraction from Bacterial Pathogens

Citation Spotlight: BugBuster® Protein Extraction Reagent for Efficient Protein Extraction from Bacterial Pathogens

BugBuster® Plus Lysonase™ Kit: Cell Lysis and Nucleic Acid Removal for Gram-negative and Gram-positive Bacteria

Cell lysis and nucleic acid removal for Gram-negative and Gram-positive bacteria using the BugBuster Plus Lysonase™ Kit

Use of BugBuster® and Lysonase™ Reagents for efficient protein extraction from Gram-positive bacteria

The combination of BugBuster® and Lysonase™ reagents greatly enhances the release of soluble proteins from Gram-positive bacteria.

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