251760

DAR-4M

A rhodamine-based photo-stable nitric oxide (NO) fluorescent indicator with a detection limit of ~10 nM.

• Synonym(s): DAR-4M, Diaminorhodamine-4M
• Empirical Formula (Hill Notation): C₂₅H₂₆N₄O₃
• Molecular Weight: 430.50
• UNSPSC Code: 12352200

Properties

• Quality Level: 100
• Assay: ≥98% (HPLC)
• form: solution
• manufacturer/tradename: Calbiochem^®
• storage condition: OK to freeze; protect from light
• color: red-violet
• fluorescence: λ_ex ~560 nm; λ_em ~575 nm
• shipped in: ambient
• storage temp.: −20°C

Description

General description

A rhodamine-based photo-stable nitric oxide (NO) fluorescent indicator with a detection limit of ~10 nM. Reacts with NO, in the presence of O₂, resulting in a triazolo-rhodamine analog (DAR-4M T) that exhibits about 840-fold greater fluorescence quantum efficiency. The fluorescence intensity is not dependent on pH, unlike DAF-2 (Cat. No. 251500).

A rhodamine-based photo-stable nitric oxide (NO) fluorescent indicator with a detection limit of ~10 nM. Reacts with NO, in the presence of oxygen, resulting in a triazolo-rhodamine analog (DAR-4M T) that exhibits about 840-fold greater fluorescence quantum efficiency. Unlike DAF-2 (Cat. No. 251500), its fluorescence intensity is not dependent on the pH. Excitation maximum: ~560 nm; emission maximum: ~575 nm.

Biochem/physiol Actions

Cell permeable: no

Primary Target
Nitric oxide (NO) fluorescent indicator

Product does not compete with ATP.

Reversible: no

Warning

Toxicity: Irritant (B)

Physical form

In DMSO.

Reconstitution

Following initial thaw, aliquot and freeze at (-20°C). Use immediately following dilution and discard any unused portion.

Other Notes

Kojima, H., et al. 2001. Anal. Chem.73, 1967.
Kojima, H., et al. 2000. Tetrahedron Lett.41, 69.

Legal Information

Not available for sale in Japan.

CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany

Safety Information

• Storage Class Code: 10 - Combustible liquids
• WGK: WGK 2
• Flash Point(F): 188.6 °F
• Flash Point(C): 87 °C