1.10283

GranuCult^® plus LB agar (MILLER)

• Synonym(s): LB Agar, LB Agar, Miller Modification, Luria Bertani Agar, Miller Luria Bertani Agar
• UNSPSC Code: 41106213

Properties

• form: medium granules (dehydrated (DCM))
• Quality Level: 200
• packaging: pkg of 500 g
• manufacturer/tradename: GranuCult^® plus
• storage condition: dry media (Keep tightly closed)
• technique(s): microbiological culture: suitable
• color: light yellow to brown
• pH: 6.8-7.2 (25 °C in H₂O, after autoclaving)
• application(s): microbiology; pharmaceutical
• storage temp.: 15-25°C
• suitability: Escherichia coli

Description

General description

GranuCult^® plus LB agar (MILLER) formulation supports the growth of E. coli. Casein peptone and yeast extracts supply essential growth factors, such as nitrogen, carbon, sulfur, minerals, and vitamins. Sodium chloride is a source of essential electrolytes and maintains osmotic equilibrium. Agar is a solidifying agent.

Application

GranuCult^® plus LB agar (MILLER) is suitable for the cultivation of E. coli in fermentation and molecular genetic studies.

Features and Benefits

• GranuCult^® offers superior granulated culture media
• Safe and sustainable due to reduced risks associated with fine dust and toxic substance inhalation, resulting in a safer work environment
• Excellent wettability, solubility, and free-flowing properties
• Convenient, with minimal component separation and clumping, even under warm or humid conditions
• High batch-to-batch reproducibility
• Prolonged shelf life of up to five years
• High number of test strains exceeding all regulatory demands
• Granulation technology allows many supplements to be included, with no need to add these separately

Linkage

Replaces: 1.10283

Analysis Note

Appearance (clearness): clear to slightly opalescent
Appearance (color): yellowish-brown
pH-value (25 °C): 6.8 - 7.2
Growth (Escherichia coli ATCC 25922 (WDCM 00013)): good
Growth (Escherichia coli ATCC 11775 (WDCM 00090)): good
Growth (Escherichia coli ATCC 53868 (DH5a)): good
Growth (Escherichia coli ATCC 39403 (JM103)): good
Growth (Escherichia coli DSM 6897 (DH5alpha)): good
Growth (Escherichia coli ATCC 47092 (BL21)): good
Incubation: 24 hours at 35 °C, aerobic

Footnote

We offer two media types: the superior granulated GranuCult^® and the cost-efficient powdered NutriSelect^® culture media, depending on your needs.
The designations basic, plus, or prime are added to indicate the quality control level, from basic quality control to standard QC plus to prime for full regulatory compliance.

Legal Information

GRANUCULT is a registered trademark of Merck KGaA, Darmstadt, Germany

NutriSelect is a registered trademark of Merck KGaA, Darmstadt, Germany

Safety Information

• Storage Class Code: 11 - Combustible Solids
• WGK: WGK 3