04-886

Anti-phospho-STAT5A/B (Tyr694/699) Antibody, clone A11W, rabbit monoclonal

culture supernatant, clone A11W, from rabbit

• Synonym(s): signal transducer and activator of transcription 5A, signal transducer and activator of transcription 5B, transcription factor STAT5B
• UNSPSC Code: 12352203
• eCl@ss: 32160702
• NACRES: NA.41

Properties

• biological source: rabbit
• Quality Level: 100
• antibody form: culture supernatant
• antibody product type: primary antibodies
• clone: A11W, monoclonal
• species reactivity: rat, chimpanzee, human, mouse, bovine
• technique(s): western blot: suitable
• isotype: IgG
• suitability: not suitable for immunoprecipitation
• NCBI accession no.: NM_003152; NM_012448
• UniProt accession no.: P42229
• shipped in: dry ice
• target post-translational modification: phosphorylation (pTyr694/pTyr699)
• Gene Information: bovine ... Stat5A(282375)

Description

General description

The STAT proteins (signal transducer and activator of transcription) comprise a family of transcription factors. STATs are activated through tyrosine phosphorylation, mainly by JAK kinases, which results in their dimerization, nuclear translocation and regulation of gene expression. STAT5 activity is induced by numerous cytokines and growth factors. While STAT5A and STAT5B share 96% amino acid homology, the two isoforms have distinct physiological functions. Phosphorylation of tyrosine 694/699 is a key marker of STAT5A/B activation.

Specificity

Phospho-STAT5A/B (Tyr694/699).

Immunogen

Epitope: pTyr694/699

KLH-conjugated, synthetic peptide containing the sequence DGpYVK in which pY corresponds to phospho-tyrosine at residue 694 of human STAT5A or residue 699 of STAT5B.

Application

Detect phospho-STAT5A/B (Tyr694/699) using this Anti-phospho-STAT5A/B (Tyr694/699) Antibody, clone A11W validated for use in WB.

Research Category
Signaling

Apoptosis & Cancer

Research Sub Category
Transcription Factors

Quality

Evaluated by western blot on RIPA lysates from 3T3/NIH cells untreated and treated with PDGF.

Western Blot Analysis:
A 1:1,000-1:2,000 dilution of this antibody detected phospho-STAT5A/B (Tyr694/699) in lysates from 3T3/NIH cells treated with PDGF.

Target description

~95 kDa

Linkage

Replaces: 05-886

Physical form

Cultured supernatant containing 0.05% sodium azide.

Storage and Stability

Stable for 1 year at -20ºC from date of receipt.
Handling Recommendations: Upon receipt, and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance.

Analysis Note

Control
PDGF treated 3T3/NIH cells

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Safety Information

• Storage Class Code: 12 - Non Combustible Liquids
• WGK: WGK 1
• Flash Point(F): Not applicable
• Flash Point(C): Not applicable