Skip to Content
Merck
All Photos(1)

Key Documents

View All Documentation

E-086

Supelco

3-epi-25-Hydroxyvitamin D3 solution

50 μg/mL in ethanol, certified reference material, Cerilliant®

Sign Into View Organizational & Contract Pricing
All Photos(1)

About This Item

Empirical Formula (Hill Notation):
C27H44O2
CAS Number:
73809-05-9
Molecular Weight:
400.64
MDL number:
MFCD11656130
UNSPSC Code:
12352205
PubChem Substance ID:
329798869
NACRES:
NA.24

grade

certified reference material

Quality Level

300

form

liquid

feature

Snap-N-Spike®/Snap-N-Shoot®

packaging

ampule of 1 mL

manufacturer/tradename

Cerilliant®

concentration

50 μg/mL in ethanol

mp

-144.0 °C

application(s)

clinical testing
clinical testing

format

single component solution

storage temp.

−20°C
−70°C

SMILES string

C[C@H](CCCC(C)(C)O)[C@H]1CC[C@H]2\C(CCC[C@]12C)=C\C=C3\C[C@H](O)CCC3=C

InChI

1S/C27H44O2/c1-19-10-13-23(28)18-22(19)12-11-21-9-7-17-27(5)24(14-15-25(21)27)20(2)8-6-16-26(3,4)29/h11-12,20,23-25,28-29H,1,6-10,13-18H2,2-5H3/b21-11+,22-12-/t20-,23-,24-,25+,27-/m1/s1

InChI key

JWUBBDSIWDLEOM-UEGFJKGBSA-N

Looking for similar products? Visit Product Comparison Guide

General description

A Certified Spiking Solution® of 3-epi-25-Hydroxyvitamin D3, the epi isomer of the prohormone 25-hydroxyvitamin D3. The presence of 3-epi-25-hydroxyvitamin D3 can affect accurate quantitation of 25-hydroxyvitamin D3 levels by most routine LC-MS/MS methods and to an unknown extent in immuno- and protein binding assays. This solution standard is suitable for critical quantitative applications in research and clinical environments including: for use in preparation of controls and calibrators for LC/UV and LC-MS/MS assays; as system tuning/suitability standards; linearity verification; internal proficiency testing; and for OEM use in preparation of reagents, development of assays and instrument IQ/OQ.

Application

3-epi-25-Hydroxyvitamin D3 may be used as a certified reference material (CRM) for the determination of the analyte in biological fluids by different chromatography techniques.

Legal Information

CERILLIANT is a registered trademark of Merck KGaA, Darmstadt, Germany
CERTIFIED SPIKING SOLUTION is a registered trademark of Cerilliant Corporation
Snap-N-Shoot is a registered trademark of Cerilliant Corporation
Snap-N-Spike is a registered trademark of Merck KGaA, Darmstadt, Germany

related product

Product No.
Description
Pricing

H-073

25-Hydroxyvitamin D2 solution, 50 μg/mL in ethanol, ampule of 1 mL, certified reference material, Cerilliant®

H-074

D6-25-Hydroxyvitamin D3 (26,26,26,27,27,27-D6) solution, 50 μg/mL in ethanol, ampule of 1 mL, certified reference material, Cerilliant®

H-083

25-Hydroxyvitamin D3 solution, 100 μg/mL in ethanol, ampule of 1 mL, certified reference material, Cerilliant®

H-089

1α,25-Dihydroxyvitamin D3 solution, 5 μg/mL in ethanol, ampule of 1 mL, certified reference material, Cerilliant®

H-090

1α,25-Dihydroxyvitamin D2 solution, 5 μg/mL in ethanol, ampule of 1 mL, certified reference material, Cerilliant®

V-026

D3-Vitamin D2 (6,19,19-D3) solution, 100 μg/mL in ethanol, ampule of 1 mL, certified reference material, Cerilliant®

V-025

Vitamin D3 solution, 1 mg/mL in ethanol, ampule of 1 mL, certified reference material, Cerilliant®

V-024

Vitamin D2 solution, 1 mg/mL in ethanol, ampule of 1 mL, certified reference material, Cerilliant®

H-107

1,25-Dihydroxyvitamin D3-13C3 (25,26,27-13C3) solution, 5 μg/mL in ethanol, ampule of 1 mL, certified reference material, Cerilliant®

H-162

1,24-Dihydroxyvitamin D3 solution, 50 μg/mL (Ethanol with 0.1%BHT (degassed)), certified reference material, ampule of 1 mL, Cerilliant®

H-160

24,25-Dihydroxyvitamin D3 solution, 50 μg/mL (Degassed Ethanol with 0.1% BHT), certified reference material, ampule of 1 mL, Cerilliant®

Pictograms

FlameExclamation mark
GHS02,GHS07

Signal Word

Danger

Hazard Statements

H225,H319

Hazard Classifications

Eye Irrit. 2 - Flam. Liq. 2

Storage Class Code

3 - Flammable liquids

WGK

WGK 1

Flash Point(F)

57.2 °F - closed cup

Flash Point(C)

14.0 °C - closed cup

Certificates of Analysis (COA)

Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.

Already Own This Product?

Find documentation for the products that you have recently purchased in the Document Library.

Visit the Document Library

A Method for Simultaneous Determination of 25-Hydroxyvitamin D3 and Its 3-Sulfate in Newborn Plasma by LC/ESI-MS/MS after Derivatization with a Proton-Affinitive Cookson-Type Reagent
Higashi T, et al.
Mass Spectrom., 5(2), S0051-S0051 (2016)
Measurement of serum 3-epi-25-hydroxyvitamin D3, 25-hydroxyvitamin D3 and 25-hydroxyvitamin D2 in infant, paediatric and adolescent populations of Korea using ultra-performance liquid chromatography-tandem mass spectrometry
Cho SE, et al.
Annals of Clinical Biochemistry, 54(5), 530-538 (2017)
Determination of four sulfated vitamin D compounds in human biological fluids by liquid chromatography--tandem mass spectrometry
Gomes FP, et al.
Journal of Chromatography. B, Biomedical Sciences and Applications, 1009(5), 80-86 (2016)

Articles

Applications of Newly Developed 2.7 μm Porous Graphitic Carbon Particles in U/HPLC

Polar graphitic carbon (2.7 μm) based Ultra/High-performance liquid chromatographic separation of four highly polar pesticides, two polar herbicides―paraquat & diquat, and Vitamin D and its metabolites.

LC/MS/MS Analysis of Vitamin D Metabolites on Ascentis® Express F5 (Monitored Ions)

The metabolic role and health implications of the various vitamin D isoforms are of current clinical interest. Therefore, it is important to have an analytical method that will resolve all of the known isoforms with necessary sensitivity and specificity

LC/MS/MS Analysis of Vitamin D Metabolites on Ascentis® Express F5

This application shows an Ascentis Express F5 provided rapid resolution of the isobaric vitamin D metabolites. MS detection provides the necessary secondary resolution.

Our team of scientists has experience in all areas of research including Life Science, Material Science, Chemical Synthesis, Chromatography, Analytical and many others.

Contact Technical Service