861030

Auramine O

Dye content 85 %, certified by the Biological Stain Commission

• Synonym(s): 4,4′-(Imidocarbonyl)bis(N,N-dimethylaniline) monohydrochloride, Basic Yellow 2, Pyoctaninum aureum
• Empirical Formula (Hill Notation): C₁₇H₂₁N₃ · HCl
• CAS Number: 2465-27-2
• Molecular Weight: 303.83
• Colour Index Number: 41000
• Beilstein: 4030061
• EC Number: 219-567-2
• MDL number: MFCD00012484
• UNSPSC Code: 12171500
• PubChem Substance ID: 24888527
• NACRES: NA.47

Properties

• grade: certified by the Biological Stain Commission
• Quality Level: 200
• form: powder or chunks
• composition: Dye content, 85%
• mp: >250 °C (dec.) (lit.)
• solubility: 95% ethanol: 1 mg/mL, clear to hazy, yellow to very deep yellow
• λmax: 370 nm; 434 nm (2nd)
• ε (extinction coefficient): ≥12000 at 249-255 nm in water at 0.005 g/L; ≥15000 at 367-373 nm in water at 0.005 g/L; ≥36000 at 429-435 nm in water at 0.005 g/L
• application(s): diagnostic assay manufacturing; hematology; histology
• storage temp.: room temp
• SMILES string: Cl[H].CN(C)c1ccc(cc1)C(=N)c2ccc(cc2)N(C)C
• InChI: 1S/C17H21N3.ClH/c1-19(2)15-9-5-13(6-10-15)17(18)14-7-11-16(12-8-14)20(3)4;/h5-12,18H,1-4H3;1H
• InChI key: KSCQDDRPFHTIRL-UHFFFAOYSA-N

Description

Application

Auramine O has been used to study the cuticle/cutin in the epidermis of the pollen presenter in Vangueria infausta.

Biochem/physiol Actions

Auramine O is used for the staining of acid-fast organisms, coccidia. Auramine O along with carbol generates brilliant yellow fluorochrome of tubercle bacilli. It is effective in detecting positive cases of tuberculosis. Auramine O binds to the mycolic acid in the bacterial cell wall.

Suitability

Certified for use by fluorescence microscopy in Churukian′s modification of Truant′s fluorescent method for acid fast bacilli on paraffin sections.

Safety Information

• Pictograms: GHS07,GHS08,GHS09
• Signal Word: Warning
• Hazard Statements: H302 - H319 - H351 - H411
• Precautionary Statements: P201 - P273 - P301 + P312 + P330 - P305 + P351 + P338 - P308 + P313
• Hazard Classifications: Acute Tox. 4 Oral - Aquatic Chronic 2 - Carc. 2 - Eye Irrit. 2
• Storage Class Code: 6.1C - Combustible, acute toxic Cat.3 / toxic compounds or compounds which causing chronic effects
• WGK: WGK 3