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Key Documents

G006129

HPLC Analysis of E. coli Lysate on Proteomix® SAX Columns, Particle Size Comparison

application for HPLC

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About This Item

technique(s)

HPLC: suitable

test parameters

column temp.: 25 °C
column: Proteomix SAX-NP10, 5 cm x 4.6 mm I.D., 10 μm particles (Z777203)
column: Proteomix SAX-NP5, 5 cm x 4.6mm I.D., 5 μm particles (Z777227)
detector: UV, 280 nm
flow rate: 0.5 mL/min
gradient: 0 - 100%B in 30 min
injection: 10 μL
mobile phase: (A) 20 mM Tris, pH 9.0; (B) A + 0.5 M NaCl
sample: 2.5 mg/mL

suitability

application for HPLC

application(s)

life science and biopharma
pharmaceutical (small molecule)

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