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Supelco

SUPELCOSIL™ LC-18-DB HPLC Column

3 μm particle size, L × I.D. 3.3 cm × 4.6 mm

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About This Item

UNSPSC Code:
41115700
eCl@ss:
32110501

Agency

suitable for USP L1

Quality Level

100

feature

endcapped

manufacturer/tradename

SUPELCOSIL™

extent of labeling

11.0% Carbon loading

parameter

0-70 °C temperature
400 bar pressure (5801 psi)

technique(s)

HPLC: suitable

L × I.D.

3.3 cm × 4.6 mm

surface area

170 m2/g

surface coverage

surface coverage 3.1 μmol/m2

matrix

silica gel, spherical particle platform

matrix active group

C18 (octadecyl) phase

particle size

3 μm

pore size

120 Å

application(s)

food and beverages

separation technique

reversed phase

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General description

SUPELCOSIL LC-DB phases are specially deactivated for basic compounds. These columns provide shorter retention, better peak shape, and higher efficiency for organic bases than can be obtained on other Type A silica reversed-phase columns.

Application

SUPELCOSIL™ LC-18-DB HPLC column may be used in the determination of 8-oxoguanine in samples of deoxyribonucleic acid (DNA) using gas chromatography coupled with mass spectrometry (GC-MS)and reversed-phase high performance liquid chromatography coupled with electrochemical detection (HPLC-EC)

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Legal Information

SUPELCOSIL is a trademark of Sigma-Aldrich Co. LLC

related product

Product No.
Description
Pricing

Storage Class Code

13 - Non Combustible Solids

WGK

WGK 3

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

Regulatory Listings

Regulatory Listings are mainly provided for chemical products. Only limited information can be provided here for non-chemical products. No entry means none of the components are listed. It is the user’s obligation to ensure the safe and legal use of the product.

EU REACH Annex XVII (Restriction List)

CAS No.

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Certificates of Analysis (COA)

Lot/Batch Number
281921-06
281921-05
281921-04
281921-03
281921-02

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Determination of 8-oxoguanine in DNA by gas chromatography-mass spectrometry and HPLC-electrochemical detection: overestimation of the background level of the oxidized base by the gas chromatography-mass spectrometry assay.
Ravanat L-J, et al.
Chemical Research in Toxicology, 8(8), 1039-1045 (1995)
Xinghua Sun et al.
Anticancer research, 25(1A), 59-62 (2005-04-09)
A rapid, sensitive and specific high-performance liquid chromatographic (HPLC) method for the separation and quantification of L-methionine in plasma has been developed. After derivatization of plasma amino acids with o-phthalaldehyde (OPA), a 50 microl sample was loaded on a reversed-phase
M Carini et al.
Journal of pharmaceutical and biomedical analysis, 18(1-2), 201-211 (1998-12-24)
A study was undertaken for the characterization and quantitative determination of the main urinary metabolites of the non-steroidal anti-inflammatory drug (NSAID) nimesulide (4-nitro-2-phenoxy-methanesulfonanilide) in man following single oral administration (200 mg). Urines were collected from six healthy volunteers at 12
P A Hynning et al.
Clinical chemistry, 34(12), 2502-2503 (1988-12-01)
A high-performance liquid chromatographic (HPLC) method for determining verapamil and norverapamil in plasma is presented and compared with gas chromatography/mass spectrometry (GC-MS). The plasma samples were extracted at alkaline pH with hexane containing 2-butanol (20 mL/L) and then back-extracted into
D C Holland et al.
Journal of AOAC International, 78(4), 1067-1071 (1995-07-01)
A liquid chromatographic (LC) method is described for the simultaneous determination of the triazine herbicides, simazine (SIM), atrazine (ATZ), and propazine (PRO) in the 12.5-100 ppb range in catfish. The herbicides are extracted from catfish homogenates with ethyl acetate, followed
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