ZRB2101

Anti-N-WASP Antibody, clone 1D16 ZooMAb^® Rabbit Monoclonal

recombinant, expressed in HEK 293 cells

• Synonym(s): Actin nucleation-promoting factor WASL, Neural Wiskott-Aldrich syndrome protein 1.
• UNSPSC Code: 12352203

Properties

• biological source: rabbit
• Quality Level: 200
• recombinant: expressed in HEK 293 cells
• conjugate: unconjugated
• antibody form: purified antibody
• antibody product type: primary antibodies
• clone: 1D16, recombinant monoclonal
• description: 1D16 Clone
• product line: ZooMAb^® learn more
• form: lyophilized
• mol wt: calculated mol wt 54.83 kDa; observed mol wt ~70 kDa
• purified by: using Protein A
• species reactivity: mouse, human, rat
• packaging: antibody small pack of 25 μL
• greener alternative product characteristics: Waste Prevention; Designing Safer Chemicals; Design for Energy Efficiency; Learn more about the Principles of Green Chemistry.
• enhanced validation: recombinant expression; Learn more about Antibody Enhanced Validation
• technique(s): immunocytochemistry: suitable; immunohistochemistry: suitable; western blot: suitable
• isotype: IgG
• epitope sequence: N-terminal half
• Protein ID accession no.: NP_003932
• UniProt accession no.: O00401
• greener alternative category: Aligned
• shipped in: ambient
• storage temp.: 2-8°C
• Gene Information: human ... WASL(8976)

Description

General description

We are committed to bringing you greener alternative products, which adhere to one or more of The 12 Principles of Green Chemistry. This antibody is Preservative-free, produced without the harm or sacrifice of animals and exceptionally stable to allow for ambient shipping and storage if needed and thus aligns with "Waste Prevention", "Designing Safer Chemicals" and "Design for Energy Efficiency". Click here for more information.

ZooMAb^® antibodies represent an entirely new generation of recombinant monoclonal antibodies. Each ZooMAb^® antibody is manufactured using our proprietary recombinant expression system, purified to homogeneity, and precisely dispensed to produce robust and highly reproducible lot-to-lot consistency. Only top-performing clones are released for use by researchers. Each antibody is validated for high specificity and affinity across multiple applications, including its most commonly used application. ZooMAb^® antibodies are reliably available and ready to ship when you need them.

Specificity

Clone 1D16 is a ZooMAb^® rabbit recombinant monoclonal antibody that specifically detects N-WASP. It targets an epitope within 15 amino acids from the N-terminal half.

Immunogen

KLH-conjugated linear peptide corresponding to 15 amino acids from the N-terminal half of human N-WASP.

Application

Quality Control Testing

Evaluated by Western Blotting in C6 cell lysate.

Western Blotting Analysis: A 1:1,000 dilution of this antibody detected N-WASP in C6 cell lysate.

Tested Applications

Western Blotting Analysis: A 1:1,000 dilution from a representative lot detected N-WASP in C2C12, MCF-7, and Neuro2A cell lysates.

Immunohistochemistry (Paraffin) Analysis: A 1:100 dilution from a representative lot detected N-WASP in human kidney tissue sections.

Immunocytochemistry Analysis: A 1:100 dilution from a representative lot detected N-WASP in MCF-7 cells.

Note: Actual optimal working dilutions must be determined by end user as specimens, and experimental conditions may vary with the end user.

Target description

Actin nucleation-promoting factor WASL (UniProt: O00401; also known as Neural Wiskott-Aldrich syndrome protein 1, N-WASP) is encoded by the WASL gene (Gene ID: 8976) in human. WASP, N-WASP, and WAVE are scaffold proteins that link cell surface signals to actin cytoskeleton. WASP expression is restricted to the non-erythroid hematopoietic cells, whereas N-WASP and WAVE are expressed ubiquitously.  WASP and N-WASP share similar protein domain organization, which comprises the WASP homolog domain (WH) at their N-terminus followed by a basic region, GTPase binding domain, a proline rich region, and a verproline-cofilin-acidic region (VCA) at its C-terminus. N-WASP is detected both in the cytoplasm and the nucleus. In the cytoplasm it regulates actin polymerization by stimulating the actin-nucleating activity of the Arp2/3 complex. In the nucleus N-WASP resides within a large nuclear protein complex containing polypyrimidine-tract-binding-protein-associated splicing factor-non-Pou-domain octamer-binding protein (PSF-NonO), nuclear actin, and RNA polymerase II.  N-WASP is reported to be preferentially localized in the cytoplasm in its phosphorylated state and in the nucleus when it is unphosphorylated. In resting cells, N-WASP is known to exist in an auto-inhibitory state that prevents the interaction between VCA region and the Arp2/3 complex. Upon binding of activate Cdc42 with GTPase binding domain N-WASP is activated due to their release from the auto-inhibitory state. This ZooMAb^® recombinant monoclonal antibody, generated by our propriety technology, offers significantly enhanced specificity, affinity, reproducibility, and stability over conventional monoclonals. (Ref.: Jain, N., and Thanabalu, T. (2015). Sci. Rep. 5; Article 15031; Wu, X.., et al. (2006). Nat. Cell Biol. 8(7); 756-763).

Physical form

Purified recombinant rabbit monoclonal antibody IgG, lyophilized in PBS, 5% Trehalose, normal appearance a coarse or translucent resin. The PBS/trehalose components in the ZooMAb formulation can have the appearance of a semi-solid (bead like gel) after lyophilization. This is a normal phenomenon. Please follow the recommended reconstitution procedure in the data sheet to dissolve the semi-solid, bead-like, gel-appearing material. The resulting antibody solution is completely stable and functional as proven by full functional testing. Contains no biocide or preservatives, such as azide, or any animal by-products. Larger pack sizes provided as multiples of 25 µL.

Reconstitution

0.3 mg/mL after reconstitution at 25 µL per vial. Please refer to guidance on suggested starting dilutions and/or titers per application and sample type.

Storage and Stability

Recommend storage of lyophilized product at 2-8°C; Before reconstitution, micro-centrifuge vials briefly to spin down material to bottom of the vial; Reconstitute each vial by adding 25 µL of filtered lab grade water or PBS; Reconstituted antibodies can be stored at 2-8°C, or -20°C for long term storage. Avoid repeated freeze-thaws.

Other Notes

Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.

Legal Information

ZooMAb is a registered trademark of Merck KGaA, Darmstadt, Germany

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Safety Information

• Storage Class Code: 11 - Combustible Solids
• WGK: WGK 1
• Flash Point(F): Not applicable
• Flash Point(C): Not applicable