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Key Documents

SRP0164

Sigma-Aldrich

Histone H4 (2-58) human

recombinant, expressed in E. coli, ≥70% (SDS-PAGE)

Synonym(s):

HIST2H4A

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About This Item

UNSPSC Code:
12352200

biological source

human

recombinant

expressed in E. coli

Assay

≥70% (SDS-PAGE)

form

aqueous solution

mol wt

32 kDa

packaging

pkg of 500 μg

storage condition

avoid repeated freeze/thaw cycles

NCBI accession no.

NM_003548

UniProt accession no.

P62805

shipped in

dry ice

storage temp.

−70°C

Gene Information

human ... HIST2H4A(8370)

General description

Human Histone 4 (GenBank Accession No. NM_003548), (2-58) with N-terminal GST-tag, MW = 32 kDa, expressed in an E. coli expression system.

Physical form

Formulated in 25 mM Tris-HCl, pH 8.0, 100 mM NaCl, 0.05% Tween-20, 20% glycerol and 3 mM DTT.

Preparation Note

Thaw on ice. Upon first thaw, briefly spin tube containing enzyme to recover full content of the tube. Aliquot enzyme into single use aliquots. Store remaining undiluted enzyme in aliquots at -70°C. Note: Enzyme is very sensitive to freeze/thaw cycles.

Certificates of Analysis (COA)

Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.

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Articles

Histone Modification and Chromatin Remodeling | Epigenetics

Epigenetic modifications are thought to occur through two key interconnected processes—DNA methylation and the covalent modification of histones.

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