S5897

Sephadex^® G-50

BioReagent, for molecular biology, DNA grade, fine

• CAS Number: 9004-54-0
• MDL number: MFCD00132771
• UNSPSC Code: 23151817
• NACRES: NA.52

Properties

• grade: DNA grade; for molecular biology
• product line: BioReagent
• form: beads
• technique(s): DNA purification: suitable
• matrix: cross-linked dextran
• swelling: 1 g swells to 9-11 mL
• bead diameter: 20-80 μm
• pore size: exclusion limit (20 bp dsDNA (rA)₂₀)
• pH: 2-10
• foreign activity: DNase, RNase, none detected
• storage temp.: room temp
• InChI: 1S/C18H32O16/c19-1-5(21)9(23)10(24)6(22)3-31-17-16(30)14(28)12(26)8(34-17)4-32-18-15(29)13(27)11(25)7(2-20)33-18/h1,5-18,20-30H,2-4H2
• InChI key: FZWBNHMXJMCXLU-UHFFFAOYSA-N

Description

General description

Sephadex^® G-50, Fine is an often-cited gel filtration medium for desalting and buffer exchange of biomolecules > 30,000 MW. Sephadex^® is prepared by crosslinking dextran with epichlorohydrin. Sephadex products differ in their degree of cross-linking and hence in their degree of swelling and their molecular fractionation range. “Fine” indicates a small particle size known for excellent efficiency and short diffusion distances.

S5897-100G′s updated product number is GE17-0573-02

Application

Sephadex^® G-50 is suitable for spin column purification of DNA (from contaminating small molecules) for use in hybridizations. It was used to purify PCR products prior to DNA sequencing to improve the sequencing quality.

Features and Benefits

• Excellent recovery
• Minimal sample dilution
• Quick and easy to use

Legal Information

Sephadex is a registered trademark of Cytiva

Safety Information

• Storage Class Code: 11 - Combustible Solids
• WGK: WGK 3
• Flash Point(F): Not applicable
• Flash Point(C): Not applicable
• Personal Protective Equipment: dust mask type N95 (US), Eyeshields, Gloves