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O5003

Sigma-Aldrich

OPI Media Supplement

Hybri-Max, γ-irradiated, powder, suitable for hybridoma

Synonym(s):

OPI Media for cell culture

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About This Item

UNSPSC Code:
12352207
NACRES:
NA.75

grade

Hybri-Max

Quality Level

sterility

γ-irradiated

form

powder

concentration

18-40 mg/vial

technique(s)

cell culture | hybridoma: suitable

impurities

endotoxin, tested

storage temp.

−20°C

Application

OPI Media Supplement has been used to grow hybridoma cell lines from XenoMouse strains and BALB/c female mice.

Other Notes

Formulated with: 0.15 g oxaloacetate, 0.05 g pyruvate, and 0.0082 g insulin.

Reconstitution

Reconstitute contents of vial with 10 mL sterile water. Do not use cell culture medium for initial reconstitution. Each vial sufficient to prepare 1 liter of medium. Final working concentration: 1 mM oxaloacetate, 0.45 mM pyruvate, 0.2 U/ml insulin.

Legal Information

Hybri-Max is a trademark of Sigma-Aldrich Co. LLC

Pictograms

CorrosionExclamation mark

Signal Word

Danger

Hazard Statements

Hazard Classifications

Eye Dam. 1 - Skin Corr. 1 - Skin Sens. 1

Storage Class Code

8A - Combustible corrosive hazardous materials

WGK

WGK 1

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable


Certificates of Analysis (COA)

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Andrew W Drake et al.
Analytical biochemistry, 328(1), 35-43 (2004-04-15)
Two biophysical methods, Biacore and KinExA, were used to kinetically and thermodynamically characterize high-affinity antigen/antibody complexes. Three to five independent experiments were performed on each platform with three different antigen/antibody complexes possessing nanomolar to picomolar equilibrium dissociation constants. By monitoring
Tzu-Ling Sung et al.
PLoS pathogens, 5(1), e1000263-e1000263 (2009-01-17)
Cyclin T1 is a regulatory subunit of a general RNA polymerase II elongation factor known as P-TEFb. Cyclin T1 is also required for Tat transactivation of HIV-1 LTR-directed gene expression. Translation of Cyclin T1 mRNA has been shown to be
Megan E Bosch et al.
Journal of neurochemistry, 148(5), 612-624 (2018-07-03)
Juvenile neuronal ceroid lipofuscinosis (JNCL) is a lysosomal storage disease caused by autosomal recessive mutations in ceroid lipofuscinosis 3 (CLN3). Children with JNCL experience progressive visual, cognitive, and motor deterioration with a decreased life expectancy (late teens-early 20s). Neuronal loss

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