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N4888

Sigma-Aldrich

Nutrient Mixture F-12 Ham

With sodium bicarbonate, without ʟ-glutamine, liquid, sterile-filtered, suitable for cell culture

Synonym(s):

Ham’s F-12

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About This Item

MDL number:
MFCD00217417
UNSPSC Code:
12352207
NACRES:
NA.75
Pricing and availability is not currently available.

Product Name

Nutrient Mixture F-12 Ham, With sodium bicarbonate, without L-glutamine, liquid, sterile-filtered, suitable for cell culture

Quality Level

400

sterility

sterile-filtered

form

liquid

technique(s)

cell culture | mammalian: suitable

impurities

endotoxin, tested

components

NaHCO3: yes
sodium pyruvate: 0.11 g/L
phenol red: yes
L-glutamine: no
HEPES: no

shipped in

ambient

storage temp.

2-8°C

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biological source

rabbit

biological source

rabbit

biological source

rabbit

biological source

rabbit

form

liquid

form

liquid

form

buffered aqueous solution

form

buffered aqueous solution

technique(s)

immunohistochemistry: 1:50-1:200, western blot: 1:500-1:2000

technique(s)

immunohistochemistry: 1:50-1:200, western blot: 1:500-1:2000

technique(s)

microarray: suitable, western blot: 1:3,000 using Jurkat human T-cell leukemia cell extract

technique(s)

immunofluorescence: 1:50-1:200, western blot: 1:500-1:2000

shipped in

wet ice

shipped in

wet ice

shipped in

dry ice

shipped in

wet ice

clone

polyclonal

clone

polyclonal

clone

polyclonal

clone

polyclonal

General description

Nutrient Mixture F-12 is suitable for the growth of primary rat hepatocytes and rat prostate epithelial cells. It is also used in a clonal toxicity assay using Chinese hamster ovary (CHO) cells.

Application

Nutrient Mixture F-12 Ham has been used:
  • in Minimum Essential Medium to maintain SH-SY5Y human neuroblastoma cells
  • to culture female-originated neuroblastoma cells
  • to culture primary retinal pigment epithelium (RPE) cells and RPE cell lines

Reconstitution

Supplement with 0.146 g/L L-glutamine.

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Storage Class Code

12 - Non Combustible Liquids

WGK

WGK 1

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

Personal Protective Equipment

dust mask type N95 (US), Eyeshields, Gloves

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    Certificates of Analysis (COA)

    Lot/Batch Number
    RNBL6335
    RNBK7756
    RNBK3282
    RNBJ6952
    RNBJ0617

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