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Key Documents

N4763

Sigma-Aldrich

Nigrosin water soluble

powder, BioReagent, suitable for cell culture

Synonym(s):

Acid black 2

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About This Item

CAS Number:
Colour Index Number:
50420
MDL number:
UNSPSC Code:
12352207
NACRES:
NA.75

product line

BioReagent

form

powder

technique(s)

cell culture | mammalian: suitable

solubility

H2O: 10 mg/mL

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General description

Nigrosin is an acidic dye, useful for negative staining, in which only the background is stained.

Application

Nigrosin water soluble has been used to stain live sperm.
Used as a viable cell staining agent especially in stem cell biology.

Storage Class Code

13 - Non Combustible Solids

WGK

WGK 3

Flash Point(C)

Not applicable


Certificates of Analysis (COA)

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Association of chromosomal aberrations and semen quality in egyptian buffalo bulls
Rushdi HE, et al.
Egyptian journal of animal production, 54(2), 95-103 (2017)
Biotechnology-II: Including Cell Biology, Genetics, Microbiology (2007)
Effect of two protocols of cryopreservation on fertilizing capacity of stallion (Equus caballus) semen
Restrepo BG, et al.
Revista Facultad Nacional de Agronomia (Medellin, Colombia), 65(2), 6711-6718 (2012)
A Novelli
Pathologica, 88(3), 188-191 (1996-06-01)
Nigrosin, an aqueous blue-black acid dye of the azine series, in conjunction with Biebrich scarlet, orange G and formic acid as mordant, it is found an excellent triple panchromatic rapid stain for histological purposes. The staining so obtained permits a
L Björndahl et al.
Human reproduction (Oxford, England), 18(4), 813-816 (2003-03-28)
The one-step eosin-nigrosin staining technique for assessment of sperm vitality was developed in the 1950s for various mammalian species. Although commonly used on human sperm in semen, a validation for this use has not previously been published. The technique was

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