MTOX1010P24

HepaRG^™ 5F Control Cells

one assay ready, 24 well plate

• UNSPSC Code: 12352207
• NACRES: NA.24

Properties

• biological source: human female liver (Source Disease: Hepatocarcinoma and Hepatitis C)
• Quality Level: 200
• form: liquid
• storage temp.: room temp

Description

General description

HepaRG is a human hepatoma cell line. The cells possess a pseudodiploid karyotype and have been characterized as an oval ductular bipotent hepatic cell line as they have the ability to differentiate into both biliary and hepatocyte lineages in the presence of DMSO. HepaRG cells express the major xenobiotic sensors (PXR, CAR and AhR), drug transporters, phase I and II drug metabolizing enzymes as well as key hepatic transcription factors involved in stress response pathways.

Application

See technical bulletin for detailed protocols

Features and Benefits

Sigma′s HepaRG 5F Clone exhibits improved growth characteristics and enhanced functionality, expressing a large panel of drug metabolism enzymes; including cytochrome P450 enzymes CYP1A2, CYP2B6, CYP2C8/9, CYP2C19, CYP2D6, CYP2E1, and CYP3A4, nuclear receptors AhR, PXR, CAR, LXR, and FXR, and phase II enzymes UGT, SULT, NAT and GST.

• The 24 well format provides delivery of ready-to-use liver cells for performing various experiments.

Quality

Tested for Mycoplasma, sterility, post-freeze viability, short terminal repeat (STR) analysis for cell line identification, cytochrome oxidase I (COI) analysis for cell line species confirmation

Legal Information

These products are covered by the License Agreement as described in Exhibit 1 and 2, in the technical bulletin.

HepaRG is a trademark of BioPredic International company

Safety Information

• Storage Class Code: 12 - Non Combustible Liquids
• WGK: WGK 3
• Flash Point(F): Not applicable
• Flash Point(C): Not applicable