M5755

MOPS-EDTA-Sodium Acetate (MESA)

powder for RNA electrophoresis buffers

• Synonym(s): MESA Buffer
• UNSPSC Code: 12161700

Properties

• grade: for molecular biology
• Quality Level: 200
• product line: BioReagent
• form: powder
• technique(s): electrophoresis: suitable
• pH: 6.8-7.2(reconstituted per directions)
• solubility: H₂O: soluble, faintly yellow
• foreign activity: DNAses, RNases, NICKases, endonucleases, and exonucleases, none detected
• storage temp.: room temp

Description

General description

MOPS – EDTA – Sodium Acetate (MESA) is the most commonly used running buffer for RNA electrophoresis prior to Northern blotting procedures. Applied voltage of 5 V/cm is recommended for maximum resolution.

Application

Suitable for use when making formaldehyde-agarose gels and associated running buffer for RNA electrophoresis.

Components

1x MESA buffer contains:

• 40 mM MOPS
• 10 mM sodium acetate
• 1 mM EDTA (pH 8.3)

Reconstitution

Dissolve entire contents of bottle in a final volume of 1L molecular biology grade water. This produces a 10x MESA stock solution that can be further diluted as needed.

Safety Information

• Storage Class Code: 11 - Combustible Solids
• WGK: WGK 3
• Flash Point(F): Not applicable
• Flash Point(C): Not applicable
• Personal Protective Equipment: dust mask type N95 (US), Eyeshields, Gloves