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Key Documents

FG0325

SAFC

Minimum Essential Medium Eagle

With Earle′s salts, L-alanyl-glutamine, and sodium bicarbonate, liquid, sterile-filtered, suitable for cell culture

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About This Item

UNSPSC Code:
12352207

Quality Level

sterility

sterile-filtered
sterile

form

liquid

technique(s)

cell culture | mammalian: suitable

impurities

Endotoxin, tested

components

stable glutamine: yes
L-glutamine: no
phenol red: yes
sodium pyruvate: no
Earle’s salts (5% CO2): yes
HEPES: no
Hanks’ salts (2% CO2): no
glucose: yes
NaHCO3: yes

shipped in

ambient

storage temp.

2-8°C

General description

Minimum Essential Medium (MEM), developed by Harry Eagle, is one of the most widely used of all synthetic cell culture media. Early attempts to cultivate normal mammalian fibroblasts and certain subtypes of HeLa cells revealed they had specific nutritional requirements that could not be met by Eagle′s Basal Medium (BME). Subsequent studies using these and other cells in culture indicated additions to BME could be made to aid growth of a wider variety of fastidious cells.

MEM, which incorporates these modifications, includes higher concentrations of amino acids so the medium more closely approximates the protein composition of cultured mammalian cells. MEM has been used for cultivation of a wide variety of cells grown in monolayers. Optional supplementation of non-essential amino acids to the formulations that incorporate either Hanks′ or Earle′s salts has broadened the usefulness of this medium.

Minimum Essential Medium (MEM), developed by Harry Eagle, is one of the most widely used of all synthetic cell culture media.

Application

Minimum Essential Medium Eagle has been used in murine fibroblast culture for in vitro cytocompatibility testing.

Other Notes

This product lacks L-Ala; L-Asn; L-Glu; Gly; L-Pro; L-Ser and L-Gln. It is supplemented with L-Ala-L-Gln dipeptide. This provides a more stable form of glutamine for cell culture. Free amino acid L-glutamine is known to be unstable in cell culture.

Storage Class Code

12 - Non Combustible Liquids

WGK

WGK 1

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable


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