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F5273

Sigma-Aldrich

Monoclonal Anti-Rat Kappa & Lambda Light Chains−FITC antibody produced in mouse

clone RT-39/RL-6, purified immunoglobulin, buffered aqueous solution

Synonym(s):

Monoclonal Anti-Rat Kappa & Lambda Light Chains

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About This Item

UNSPSC Code:
12352203
NACRES:
NA.46

biological source

mouse

conjugate

FITC conjugate

antibody form

purified immunoglobulin

antibody product type

secondary antibodies

clone

RT-39/RL-6, monoclonal

form

buffered aqueous solution

mol wt

antigen 25 kDa

storage condition

protect from light

technique(s)

dot immunobinding: 1:40
immunohistochemistry (formalin-fixed, paraffin-embedded sections): 1:40

isotype

IgG1/IgG2a

shipped in

dry ice

storage temp.

−20°C

target post-translational modification

unmodified

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General description

The product is a mixture derived from hybridomas RT-39 and RL-6, secreting monoclonal antibodies against rat κ and λ light chains, respectively.
The product recognizes epitopes located on the rat κ and λ light chains on the various rat immunoglobulin classes and subclasses. The epitope recognized on the κ light chains is shared by both the 1a and 1b allotypes of this molecule. The antibody detects the κ and λ light chains derived from normal serum immunoglobulins or from myeloma proteins. Weak cross-reaction is observed with guinea pig immunoglobulins but not with human, bovine, cat, chicken, dog, goat, horse, mouse, pig, rabbit or sheep immunoglobulins.

Application

Monoclonal Anti-Rat Kappa & Lambda Light Chains-FITC antibody produced in mouse has been used in:
  • fluorescent dot binding immunoassay (FDIBA)
  • immunocytochemistry
  • immunohistochemistry
  • flow cytometry

Biochem/physiol Actions

FITC Monoclonal Anti-Rat kappa and lambda Light Chains may serve as an essential tool in the localization of light chains on rat immunoglobulins.

Physical form

Solution in 0.01 M phosphate buffered saline, pH 8.0, containing 1% bovine serum albumin and 15 mM sodium azide.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

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Storage Class Code

10 - Combustible liquids

WGK

nwg

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

Personal Protective Equipment

dust mask type N95 (US), Eyeshields, Gloves

Certificates of Analysis (COA)

Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.

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M M Aguilar et al.
The Journal of trauma, 45(1), 14-18 (1998-07-29)
T-cell response to trauma has been assessed primarily by sampling peripheral blood lymphocytes. We hypothesized that lymphocytes residing in tissue and traveling through lymph vessels are more likely to be activated by tissue injury and hemorrhage-induced hypoperfusion. We compared peripheral

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