CLS3330
Corning® CellBIND® Surface CellSTACK® cell culture chambers
CellBIND® surface, polystyrene, sterile, 1 Chamber with Vent Caps, graduations, orange high-density polyethylene cap, case of 8
Synonym(s):
cell culture flasks, scale up cell culture flasks, tissue culture flasks
About This Item
Recommended Products
material
orange high-density polyethylene cap
polystyrene
rectangular bottom flask
short neck
sterility
sterile
feature
cap vented
graduations
packaging
pack of 1
case of 8
manufacturer/tradename
Corning 3330
surface area
636 cm2
working volume
125-190 mL
suitability
suitable for (scale-up cell culture)
binding type
CellBIND® surface
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General description
Both enzymatic and non-enzymatic dissociating solutions have been used to remove and harvest cells from CellBIND surfaces, scraping is not recommended due to the increased strength of cell attachment.
CellSTACK chambers are ideal for cell culture scale-up, providing cell yields in the 107 - 109 range and are useful alternatives to multiple roller bottles or spinner flasks.
- Ideal for cell culture scale-up
- useful alternatives to multiple roller bottles or spinner flasks
- High mechanical strength and structural integrity
- 100% leak tested
- Certified nonpyrogenic and sterilized by gamma irradiation, with low particulate contamination from modern manufacturing methods
- Easy to use larger openings with threaded closures and vented caps
- Filling ports allow direct access to chamber bottom, with more options for sterile transfer of media and cultures
Features and Benefits
- Has 636cm2 cell growth area
- Two 26mm diameter filling ports allow direct access to chamber bottom providing greater flexibility for sterile filling and emptying by pouring, pipetting or via tubing in a fully closed system
- Standard 33mm threaded caps have 0.2μm pore nonwettable membranes sealed directly to the caps to allow gas exchange while minimizing the risk of contamination
- Optional 33mm threaded caps are available with integrally sealed USP Class VI certified C-FLEX tubing to allow direct sterile transfer of media and cells
- USP Class VI polystyrene provides excellent optical clarity and mechanical strength
- Sterilized by gamma radiation and certifiied nonpyrogenic
- 100% leak tested prior to shipping
- Treated with a CellBIND surface treatment for enhanced cell attachment.
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Protocols
This technical article covers the protocol for how to properly harvest cells from Corning® CellSTACK® culture chambers.
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