CLLS1014

A549 Cells HIF1A -/-/-

human male lung (Source Disease: Lung carcinoma)

• UNSPSC Code: 41106514
• NACRES: NA.81

Properties

• product name: A549 Cells HIF1A -/-/-,
• biological source: human male lung (Source Disease: Lung Carcinoma)
• OMIM accession no.: 603348
• storage temp.: −196°C
• Gene Information: human ... HIF1a(3091)

Description

General description

A549 Cells HIF1A -/-/- are lung carcinoma, epithelial cells, from a human caucasian male (aged 58 years), with a ZFN knock out modification.

This product corresponds to ATCC Cat. No. CCL-185

Application

Media Renewal changes two to three times per week

Rapidly thaw vial by gentle agitation in 37°C water bath (~2 minutes), keeping vial cap out of the water. Decontaminate with 70% ethanol, add 9 mL culture media and centrifuge 125 x g (5-7 minutes). Resuspend in complete culture media and incubate at 37°C in a 5% CO2 atmosphere.

Subculture Ratio: approx. 1:3 to 1:8

The base medium for this cell line is F12 Ham Medium, Cat. No. N4888. To make the complete growth medium, add the following components to the base medium: fetal bovine serum, Cat. No. F4135, to a final concentration (v/v) of 10% and L-glutamine, Cat. No. G7513, at a final concentration of 2mM.

The cell freezing medium is DMSO 1X (Cat. No. C6164)

Features and Benefits

These A549 cells are adherent, with a doubling time of approx. 22 hours.

Zinc finger nuclease (ZFN) knock out on chromosome 14q23.2

Quality

Tested for Mycoplasma, sterility, post-freeze viability, short terminal repeat (STR) analysis for cell line identification, PCR assay for cell line species confirmation.

Disclaimer

RESEARCH USE ONLY. This product is regulated in France when intended to be used for scientific purposes, including for import and export activities (Article L 1211-1 paragraph 2 of the Public Health Code). The purchaser (i.e. enduser) is required to obtain an import authorization from the France Ministry of Research referred in the Article L1245-5-1 II. of Public Health Code. By ordering this product, you are confirming that you have obtained the proper import authorization.

Kit Components Only

• A549 Cells HIF1A -/-/- 1 vial

Kit Components Also Available Separately

• A549 Control Cells 1 vial