KYSE-410

94072023, human esophagus, Epitheloid

• Synonym(s): KYSE0410 Cells, KYSE410 Cells
• UNSPSC Code: 41106514

Properties

• product name: KYSE-410, 94072023
• biological source: human esophagus
• growth mode: Adherent
• karyotype: Hypotetraploid with 4% polyploidy
• morphology: Epitheloid
• products: Not specified
• receptors: Not specified
• technique(s): cell culture | mammalian: suitable
• relevant disease(s): cancer
• shipped in: dry ice
• storage temp.: −196°C

Description

Cell Line Origin

Human oesophageal squamous cell carcinoma

Cell Line Description

Established from the poorly differentiated invasive oesophageal squamous cell carcinoma resected from the cervical oesophagus of a 51-year old Japanese man prior to treatment. Reported to overexpress heparin binding growth factor (hst-1) and cyclin D. This cell line has been mycoplasma eradicated at ECACC. By short tandem repeat (STR)-PCR analysis the Y chromosome could not be detected in this cell line. It is a known phenomenon that SSC cell lines can lose their Y chromosome.

DNA Profile

STR-PCR Data: Amelogenin: X
CSF1PO: 12
D13S317: 11
D16S539: 10,12
D5S818: 13
D7S820: 12
THO1: 8
TPOX: 8,11
vWA: 16,18

Culture Medium

RPMI 1640 + 2mM Glutamine + 5-10% Foetal Bovine Serum (FBS)

Subculture Routine

Split confluent cultures 1:5 to 1:10 twice a week i.e. seeding at 2-4x10,000 cells/cm² using trypsin/EDTA;5% CO₂; 37°C. Doubling time ~45h

Other Notes

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