K1 Cell Line human

92030501

• UNSPSC Code: 41106514

Properties

• biological source: human thyroid
• description: Human thyroid carcinoma
• growth mode: Adherent
• karyotype: Not specified
• morphology: Not specified
• products: Wild-type p53 tumour suppressor gene
• receptors: Not specified
• technique(s): cell culture | mammalian: suitable
• relevant disease(s): cancer
• shipped in: dry ice
• storage temp.: −196°C

Description

Cell Line Origin

Human thyroid carcinoma

Cell Line Description

Derived from a primary papillary thyroid carcinoma. Retains thyroid follicular cell differentiation e.g. thyroglobulin synthesis. Expresses wild-type p53 tumour suppresser gene. It has been reported that K1 cells have their origin in the thyroid papillary carcinoma cell line GLAG-66.

Application

K1 Cell Line human has also been used to study and correlate the expression levels of c-KIT (a receptor tyrosine kinase) and two markers of thyrocyte differentiation (PAX8 and TTF-1).

Tumourigenicity studies, particularly the role of p53

DNA Profile

STR-PCR Data: Amelogenin: X,Y
CSF1PO: 11,12
D13S317: 11,14
D16S539: 11,12
D5S818: 10,11
D7S820: 11
THO1: 6,9
TPOX: 8
vWA: 17,18

Culture Medium

DMEM:Ham′s F12:MCDB 105 (2:1:1) + 2mM Glutamine + 10% Foetal Bovine Serum (FBS).

Subculture Routine

Split sub-confluent cultures (70-80%) 1:2 to 1:4 i.e. seeding at 2x1,000,000 cells/cm² using 0.25% trypsin/EDTA; 5% CO₂; 37°C.

Other Notes

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