Skip to Content
Merck
All Photos(1)

Key Documents

HT29 gluc C1

92012401, human colon, Epithelial

Sign Into View Organizational & Contract Pricing


About This Item

UNSPSC Code:
41106514

product name

HT29 gluc C1, 92012401

biological source

human colon

growth mode

Adherent

karyotype

Not specified

morphology

Epithelial

products

Elevated levels of cystic fibrosis transmembrane conductance regulator (CFTR) protein

receptors

Not specified

technique(s)

cell culture | mammalian: suitable

relevant disease(s)

cancer

shipped in

dry ice

storage temp.

−196°C

Cell Line Origin

Human Caucasian colon adenocarcinoma

Cell Line Description

A subclone of the parent HT29-18 differentiated to specific cell types for studies on cystic fibrosis.

Culture Medium

DMEM + 2mM Glutamine + 1mM Sodium Pyruvate (NaP) + 44mM Sodium Bicarbonate (NaHCO3) + 10mg/l Transferrin + 10% Foetal Bovine Serum (FBS).

Subculture Routine

Split sub-confluent cultures (70-80%) 1:2 to 1:8, i.e. seeding at 1-4x10,000 cells/cm2 using 0.25% trypsin or trypsin/EDTA; 5% CO2; 37°C.

Other Notes

Additional freight & handling charges may be applicable for Asia-Pacific shipments. Please check with your local Customer Service representative for more information.

Certificates of Analysis (COA)

Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.

Already Own This Product?

Find documentation for the products that you have recently purchased in the Document Library.

Visit the Document Library

Our team of scientists has experience in all areas of research including Life Science, Material Science, Chemical Synthesis, Chromatography, Analytical and many others.

Contact Technical Service