Recommended Products
product name
NCTC clone 1469, 88111403
biological source
mouse liver
growth mode
Adherent
karyotype
Not specified
morphology
Epithelial
products
Not specified
receptors
Not specified
technique(s)
cell culture | mammalian: suitable
shipped in
dry ice
storage temp.
−196°C
Cell Line Origin
Mouse C3H/An liver
Cell Line Description
Derived from mouse liver cell line NCTC 721 which was obtained from normal liver of a 2 day old C3H/An mouse. The cells have been used in biochemical and nutritional studies. The cells are susceptible to VSV (Indiana strain).
Application
Cell suspension systems. Biochemical, nutritional and virus studies. VSV(Indiana)
Culture Medium
NCTC 135 + 2mM Glutamine + 10% Horse Serum (HS)
Subculture Routine
Split sub-confluent cultures (70-80%) 1:2 to 1:4 using only mechanical techniques, such as scraper, do not use trypsin, 5-10CO2; 37°C. Change media every 3 days. Freeze in 10% glycerol/90% FBS. Cells are shed into the medium giving it a turbid appearan
Other Notes
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Certificates of Analysis (COA)
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