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Key Documents

LTK-

85011432, mouse connective tissue, Fibroblast

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About This Item

UNSPSC Code:
41106514

product name

LTK-, 85011432

biological source

mouse connective tissue

growth mode

Adherent

karyotype

Not specified

morphology

Fibroblast

products

Not specified

receptors

Not specified

technique(s)

cell culture | mammalian: suitable

shipped in

dry ice

storage temp.

−196°C

Cell Line Origin

Mouse C34/An connective tissue

Cell Line Description

Sub-line of BUdR resistant strain of the L-M mouse line (Sigma Catalogue number. 87032401), which in turn was derived from L929 (Sigma Catalogue number. 85011425). TK- cells cannot grow in HAT medium. Stable TK- mutants are only maintained if the cells are kept for several passages in growth medium containing BUdR.

Application

Somatic cell hybridisation and gene transfer studies

Culture Medium

DMEM + 2mM Glutamine + 10% Foetal Bovine Serum (FBS).

Subculture Routine

Split semi-confluent cultures seeding at 5x1,000 to 2x10,000 cells/cm2 using 0.25% trypsin or trypsin/EDTA; 5% CO2; 37°C.

Other Notes

Additional freight & handling charges may be applicable for Asia-Pacific shipments. Please check with your local Customer Service representative for more information.

Certificates of Analysis (COA)

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