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biological source
Rat x Rat Hybridoma
ImmunoDon
DA rat spleen
Myeloma
Y3.AG.1.2.3
Reactivity
Human IL2 receptor alpha (CD25)
technique(s)
cell culture | mammalian: suitable
isotype
IgG2b
shipped in
dry ice
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Cell Line Origin
Monoclonal antibody, mouse CD4 epitope A
Cell Line Description
Rat hybridoma producing antibody against human CD25. The designation HL indicates that it has bselected for loss of the original Y3 myeloma derived (non-specific) light chain. Cambridge Enterprise Reference number AB61:www.enterprise.cam.ac.uk.
Immunogen
Activated human T cells
Culture Medium
IDMEM (I3390) + 2 mM L-Glutamine (G7513) + 5% FBS / FCS (F2442). Where the cells are grown under static conditions, 0.02% EDTA, Trypsin (T4424) or mechanical agitation maybe required to dislodge them from the plastic.
Subculture Routine
Maintain cultures between 3-9 x 105 cells/ml. 5% CO2; 37 °C. Saturation density 9 x 105/ml.
Other Notes
Additional freight & handling charges may be applicable for Asia-Pacific shipments. Please check with your local Customer Service representative for more information.
Disclaimer
This cell line has special release conditions: Commercial organisations are required to complete the ′Cell Line Release Authorisation for Research Use in Commercial Organisations′ release conditions form.
Certificates of Analysis (COA)
Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.
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