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C50120

Sigma-Aldrich

CM Sephadex® C-50

Synonym(s):

CM Sephadex®, Carboxymethyl Sephadex®

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About This Item

CAS Number:
MDL number:
UNSPSC Code:
47101511
NACRES:
NA.56

form

powder

technique(s)

activity assay: suitable

bead size

40-125 μm (dry)

pore size

~200,000 Da exclusion limit

operating pH

6-10

capacity

4.0-5.0 meq/g ion exchange capacity

compatibility

mode of use weak cation exchange chromatography

General description

C50120-100G′s updated product number is GE17-0220-01

Application

Sephadex C-50 is used for cation exchange media, separation media, resins, ion exchange chromatography, protein chromatography and affinity chromatography. Sephadex® C-50 has been used to study biochemical characteristics of cobra venom and effects of vitamins on lactoperoxidase enzyme activity.

Legal Information

Sephadex is a registered trademark of Cytiva

Storage Class Code

11 - Combustible Solids

WGK

WGK 3

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

Personal Protective Equipment

dust mask type N95 (US), Eyeshields, Gloves

Certificates of Analysis (COA)

Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.

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Anil S Thakur et al.
PloS one, 2(10), e1091-e1091 (2007-11-01)
Crystallization is a major bottleneck in the process of macromolecular structure determination by X-ray crystallography. Successful crystallization requires the formation of nuclei and their subsequent growth to crystals of suitable size. Crystal growth generally occurs spontaneously in a supersaturated solution
Human liver acid phosphatases: purification and properties of a low-molecular-weight isoenzyme.
E M Taga et al.
Archives of biochemistry and biophysics, 214(2), 505-515 (1982-04-01)
N J Abbott et al.
Journal of cell science, 103 ( Pt 1), 23-37 (1992-09-01)
Primary culture of rat brain endothelial cells is described, based on the method of C. C. W. Hughes and P. L. Lantos. The cells have been characterised using morphological and immunocytochemical techniques, and systematic studies undertaken to determine the optimal
Melda Şişecioğlu et al.
Journal of enzyme inhibition and medicinal chemistry, 25(6), 779-783 (2010-02-04)
Melatonin is the chief secretory product of the pineal gland and is synthesized enzymatically from serotonin. These indoleamine derivatives play an important role in the prevention of oxidative damage. Lactoperoxidase (LPO; EC 1.11.1.7) was purified from bovine milk with three
K Cline et al.
Plant physiology, 68(1), 207-220 (1981-07-01)
The fact that fungal glucans will stimulate soybeans to accumulate phytoalexins prompted an investigation of soybean cell beta-1,3-glucanases and beta-glucosidases, as well as the ability of these enzymes to hydrolyze the fungal glucans. Several beta-1,3-glucanases and beta-glucosidases can be solubilized

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