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C-28019

Sigma-Aldrich

Mesenchymal Stem Cell Growth Medium DXF

Ready-to-use kit including Basal Medium and SupplementMix, 500 ml

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About This Item

UNSPSC Code:
12352207
NACRES:
NA.71

form

liquid

packaging

pkg of 500 ml

technique(s)

cell culture | mammalian: suitable

shipped in

wet ice

General description

MSC Growth Medium XF 500 ml, ready-to-use, with C-39819 attached to flask. Serum-free and xeno-free cell culture medium for proliferation of mesenchymal stem cells. Fibronectin coating required.

Application

The Mesenchymal Stem Cell Growth Medium DXF is a defined and xeno-free culture medium for routine culture of multipotent human Mesenchymal Stem Cells. Rigorous quality control tests ensure reliable and consistent product quality.Recommended for:Human Mesenchymal Stem Cells from Bone Marrow (hMSC-BM)Human Mesenchymal Stem Cells from Umbilical Cord Matrix (hMSC-UC)Human Mesenchymal Stem Cells from Adipose Tissue (hMSC-AT)Note: MSC Growth Medium DXF does not contain attachment- and spreading factors. Therefore, culture vessels must be precoated with 10 μg/ml human Fibronectin according to the instruction manual.

Quality

All lots of PromoCell Mesenchymal Stem Cell Media are subjected to comprehensive quality control tests using primary human mesenchymal stem cells. Each lot of MSC Growth Medium is checked for maintenance of multipotency, growth promoting activity, adherence rate, and typical morphology of the tested mesenchymal stem cells. In addition, all lots of media have been tested for the absence of microbial contaminants (fungi, bacteria, mycoplasma).

Storage and Stability

Store the Basal Medium at 4 to 8°C in the dark and the SupplementMix at -20°C immediately after arrival. Do not freeze the Basal Medium. If stored properly, the products are stable until the expiry date stated on the label. After adding the supplements to the Basal Medium, the complete MSC Growth Medium DXF is stable for 3 weeks at 4 to 8°C. Do not freeze the complete medium. For use, pre-warm only an aliquot of the complete medium and keep the remaining medium refrigerated at 4 to 8°C.

Subculture Routine

Click here for more information.

Storage Class Code

10 - Combustible liquids

Certificates of Analysis (COA)

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Articles

Human Mesenchymal Stem Cells and Growth/Differentiation Media

Mesenchymal Stem Cells and Media were developed for the in vitro expansion and directed differentiation of mesenchymal stem cells (MSC) from bone marrow, the umbilical cord matrix (Wharton´s Jelly) and adipose tissue. Protocols for handling, cell passaging, media, and product use.

Neuronal Mesenchymal Stem Cells

Protocol to differentiate human mesenchymal stem cells into neurons with extensive dendrites and axons in 3 days. Explore over 350 PromoCell products on Sigma.com.

Mesenchymal Stem Cell Characterization

Are your mesenchymal stem cells really MSCs? Learn more about the MSC characterization guidelines proposed by the International Society for Cellular Therapy (ISCT).

Protocols

Human Mesenchymal Stem Cells and Growth/Differentiation Media

Mesenchymal Stem Cells and Media were developed for the in vitro expansion and directed differentiation of mesenchymal stem cells (MSC) from bone marrow, the umbilical cord matrix (Wharton´s Jelly) and adipose tissue. Protocols for handling, cell passaging, media, and product use.

Mesenchymal Stem Cell Culture Protocols

Information about mesenchyme, specifically mesenchymal stem cell procotols. Step-by-step cell culture protocols for mesenchymal stem cell (MSC) isolation, expansion and differentiation.

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