A8706
Avidin from egg white
recombinant, expressed in corn, ≥12 units/mg protein (E1%/280)
Synonym(s):
AVID_CHICK
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About This Item
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recombinant
expressed in corn
Quality Level
Assay
≥80% protein basis (biuret)
form
powder
specific activity
≥12 units/mg protein (E1%/280)
mol wt
glycoprotein 66 kDa
subunit 16 kDa
technique(s)
ELISA: suitable
immunoblotting: suitable
immunoelectrophoresis: suitable
UniProt accession no.
storage temp.
2-8°C
Gene Information
chicken ... AVD(396260)
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General description
Avidin is a homotetrameric glycoprotein found in the egg white of birds, reptiles and amphibians. Each subunit is 16 kDa, singly glycosylated and binds to a molecule of biotin with greater affinity and specificity. Recombinant avidin from corn is similar to avidin from egg white in terms of properties like isoelectric point (pI) and antigenic properties except for the molecular weight. Avidin from corn has low molecular weight than chicken egg-derived avidin. This might be due to less complex glycosylation composition in plants. Commercial production of avidin from corn has certain advantages in terms of availability of greater biomass and avoiding the co-purification of animal virus.
Application
Avidin egg white was used in an assay using functionalized xenon as a biosensor to detect biotin-avidin binding. Egg white was used at 80 nmol.
Avidin forms an extremely strong complex with d-biotin (Kd ~ 10-15). This activity has made both avidin and biotin extremely useful labels in immunochemical methods of detection and quantitation.
Unit Definition
One unit will bind 1.0 μg of d-biotin.
Preparation Note
Affinity purified
Storage Class Code
11 - Combustible Solids
WGK
WGK 3
Flash Point(F)
Not applicable
Flash Point(C)
Not applicable
Personal Protective Equipment
dust mask type N95 (US), Eyeshields, Gloves
Certificates of Analysis (COA)
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