VP001
VirusExpress® 293T Lentiviral Production Cells
Pharma Manufacturing
Synonym(s):
HEK293T cells for lentivirus production
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General description
VirusExpress 293T Lentiviral Production Cells are derived from the HEK293T cell line (ATCC CRL-3216) through single cell cloning. The parental HEK293 cell line was established by transformation of human embryonic kidney cells with sheared human adenovirus 5 DNA (Graham FL et al. 1977). A 4-kb adenoviral genome fragment is known to have integrated into chromosome 19 and encodes for E1A/E1B proteins. HEK293T cells were created by stable transfection of the HEK293 cell line with a temperature-sensitive mutant of SV40 large T antigen. Due to the expression of the large T antigen, transfected plasmids that carry the SV40 origin of replication can replicate in 293T cells and will transiently maintain a high copy number. Our clonal derivative eliminates variability from the population while also being adapted to suspension, serum-free conditions. Suspension culture and chemically defined medium allow scalability into stirred tank bioreactors for yields that can meet commercial needs.
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Storage Class Code
12 - Non Combustible Liquids
WGK
WGK 1
Flash Point(F)
Not applicable
Flash Point(C)
Not applicable
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A transfection-based solution to viral vector production using a suspension cell line, chemically defined medium, and a process with proven performance at scale.
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A transfection-based solution to viral vector production using a suspension cell line, chemically defined medium, and a process with proven performance at scale.
A transfection-based solution to viral vector production using a suspension cell line, chemically defined medium, and a process with proven performance at scale.
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