D0531

5-([4,6-Dichlorotriazin-2-yl]amino)fluorescein hydrochloride

≥90% (HPLC)

• Synonym(s): DTAF
• Empirical Formula (Hill Notation): C₂₃H₁₂Cl₂N₄O₅ · HCl
• CAS Number: 21811-74-5
• Molecular Weight: 531.73
• MDL number: MFCD00051407
• UNSPSC Code: 12171500
• PubChem Substance ID: 24893242
• NACRES: NA.47

Properties

• Quality Level: 200
• Assay: ≥90% (HPLC)
• form: powder
• solubility: methanol: 20 mg/mL, clear to slightly hazy, yellow to orange
• fluorescence: λ_ex 495 nm; λ_em 516 nm
• application(s): diagnostic assay manufacturing; hematology; histology
• storage temp.: 2-8°C
• SMILES string: Cl.Oc1ccc2c(Oc3cc(O)ccc3C24OC(=O)c5cc(Nc6nc(Cl)nc(Cl)n6)ccc45)c1
• InChI: 1S/C23H12Cl2N4O5.ClH/c24-21-27-22(25)29-23(28-21)26-10-1-4-13(16(7-10)20(32)33)19-14-5-2-11(30)8-17(14)34-18-9-12(31)3-6-15(18)19;/h1-9,30H,(H,32,33)(H,26,27,28,29);1H
• InChI key: CEKSHCAIHGNWBK-UHFFFAOYSA-N

Description

Application

5-([4,6-Dichlorotriazin-2-yl]amino)fluorescein hydrochloride has been used for labeling of yeast cells to study the adhesion of yeast cells to the digestive tract of sea bass (Dicentrarchus labrax) larvae and leopard grouper (Mycteroperca rosacea). It has also been used to label bacteria (Vibrio spp.).
5-([4,6-Dichlorotriazin-2-yl]amino)fluorescein hydrochloride has been used to covalently label xanthan gum (XG) to study its gelatin behavior.

A fluorescein derivative which reacts with amino groups and N-terminal amino acids of proteins at neutral pH.

Safety Information

• Storage Class Code: 11 - Combustible Solids
• WGK: WGK 3
• Flash Point(F): Not applicable
• Flash Point(C): Not applicable
• Personal Protective Equipment: dust mask type N95 (US), Eyeshields, Gloves