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B7920

Sigma-Aldrich

Brilliant Blue R

≥70% purity (HPLC), powder

Synonym(s):

Acid Blue 83, Brilliant indocyanin 6B, Coomassie Brilliant Blue R

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About This Item

Empirical Formula (Hill Notation):
C45H44N3NaO7S2
CAS Number:
Molecular Weight:
825.97
Colour Index Number:
42660
Beilstein:
5718025
EC Number:
MDL number:
UNSPSC Code:
12171500
PubChem Substance ID:
NACRES:
NA.47

product name

Brilliant Blue R, pure

Quality Level

Assay

≥70% (HPLC)

form

powder

solubility

H2O: 1 mg/mL

λmax

586-592 nm in ethanol

application(s)

diagnostic assay manufacturing
hematology
histology

storage temp.

room temp

SMILES string

[Na+].CCOc1ccc(Nc2ccc(cc2)C(\c3ccc(cc3)N(CC)Cc4cccc(c4)S([O-])(=O)=O)=C5\C=C/C(C=C5)=[N+](\CC)Cc6cccc(c6)S([O-])(=O)=O)cc1

InChI

1S/C45H45N3O7S2.Na/c1-4-47(31-33-9-7-11-43(29-33)56(49,50)51)40-23-15-36(16-24-40)45(35-13-19-38(20-14-35)46-39-21-27-42(28-22-39)55-6-3)37-17-25-41(26-18-37)48(5-2)32-34-10-8-12-44(30-34)57(52,53)54;/h7-30H,4-6,31-32H2,1-3H3,(H2,49,50,51,52,53,54);/q;+1/p-1

InChI key

NKLPQNGYXWVELD-UHFFFAOYSA-M

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Application

Brilliant Blue R has been used in staining of gels after electrophoresis. It has also been used to determine protein in the protein bodies.
For detection of protein bands following electrophoresis.

Biochem/physiol Actions

Brilliant Blue R is a methanol based stain. It can also stain agarose gels. It consists of methanol and acetic acid.

Legal Information

Storage Class Code

11 - Combustible Solids

WGK

WGK 3

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

Personal Protective Equipment

dust mask type N95 (US), Eyeshields, Gloves

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