Skip to Content
Merck
All Photos(2)

Documents

70186

Millipore

EMB Agar

NutriSelect® Plus, suitable for microbiology

Synonym(s):

Eosin Methylene Blue Agar

Sign Into View Organizational & Contract Pricing
All Photos(2)

About This Item

UNSPSC Code:
41171606
NACRES:
NA.74

sterility

non-sterile

Quality Level

200

form

powder

shelf life

limited shelf life, expiry date on the label

composition

agar, 15.0 g/L
dipotassium hydrogen phosphate, 2.0 g/L
eosin Y, 0.4 g/L
lactose, 10.0 g/L
methylene blue, 0.065 g/L
peptone, 10.0 g/L

manufacturer/tradename

NutriSelect® Plus

technique(s)

microbiological culture: suitable

final pH

7.1±0.2 (25 °C)

application(s)

agriculture
cleaning products
clinical testing
cosmetics
environmental
food and beverages
personal care
microbiology

suitability

slightly selective and differential for Candida spp.
slightly selective and differential for Citrobacter spp.
slightly selective and differential for Enterobacter spp.
slightly selective and differential for Enterococcus spp.
slightly selective and differential for Escherichia coli
slightly selective and differential for Klebsiella spp.
slightly selective and differential for Proteus spp.
slightly selective and differential for Salmonella spp.
slightly selective and differential for Staphylococcus spp.
slightly selective and differential for coliforms
slightly selective and differential for enterobacteriaceae

Related Categories

Application

A versatile solid medium for the differentiation of Eschericha coli and Aerobacter aerogenes, for identifcation of Candida albicans and for the identification of coagulase-positive Staphylococci.

Preparation Note

Suspend 37.5 g in 1 litre of distilled water. Bring to the boil to dissolve completely, sterilize by autoclaving at 121°C for 15 minutes. Cool to 60°C and shake the medium in order to oxidize the methylene blue and to suspend the precipitate.

Other Notes

Recommended by the American Public Health Association (APHA) and the United States Pharmacopeia (USP); Compendium of Methods for the Microbiological Examination of Foods; Enumeration of E. coli from lake water.

Footnote

We offer two media types: the superior granulated GranuCult® and the cost-efficient powdered NutriSelect® culture media, depending on your needs.
The designations basic, plus, or prime are added to indicate the quality control level, from basic quality control to standard QC plus to prime for full regulatory compliance.

Legal Information

GRANUCULT is a registered trademark of Merck KGaA, Darmstadt, Germany
NutriSelect is a registered trademark of Merck KGaA, Darmstadt, Germany

Pictograms

Exclamation mark
GHS07

Signal Word

Warning

Hazard Statements

H317

Hazard Classifications

Skin Sens. 1

Storage Class Code

11 - Combustible Solids

WGK

WGK 3

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

Personal Protective Equipment

dust mask type N95 (US), Eyeshields, Gloves

Choose from one of the most recent versions:

Certificates of Analysis (COA)

Lot/Batch Number
BCCG1812
BCCD6329
BCCB6959
BCCB6968
BCBW6831

Don't see the Right Version?

If you require a particular version, you can look up a specific certificate by the Lot or Batch number.

Search for a COA

Already Own This Product?

Find documentation for the products that you have recently purchased in the Document Library.

Visit the Document Library

M. Levine
The Journal of Infectious Diseases, 23, 43-43 (1918)
Y Henis et al.
Microbial ecology, 17(2), 171-180 (1989-03-01)
The population of a strain ofEscherichia coli that was resistant to nalidixic acid and streptomycin declined rapidly in samples of sterile and nonsterile Cayuga Lake water and reached an undetectable level in nonsterile water at 24 and 72 hours when
J.E. Holt-Harris et al.
The Journal of Infectious Diseases, 18, 596-596 (1916)
Shuaiguo Zhao et al.
Lab on a chip, 19(6), 941-947 (2019-02-01)
Microfluidic-based portable devices for stool analysis are important for detecting established biomarkers for gastrointestinal disorders and understanding the relationship between gut microbiota imbalances and various health conditions, ranging from digestive disorders to neurodegenerative diseases. However, the challenge of processing stool
Isolation and identification of coagulase-positive Staphylococci on Levine's eosin-methylene blue agar.
N J MENOLASINO
The Journal of laboratory and clinical medicine, 56, 908-910 (1960-12-01)

Articles

Yeast

Yeast is one of the most important microorganisms known and utilised by mankind. Ancient Middle Eastern civilisations used the organism to bake bread and to produce mead, beer and wine.

Selective Growth Media for Differentiation and Detection of Escherichia coli and Other Coliforms

An article concerning selective growth media for differentiation and detection of Escherichia coli and other coliforms.

Our team of scientists has experience in all areas of research including Life Science, Material Science, Chemical Synthesis, Chromatography, Analytical and many others.

Contact Technical Service