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Roche

Anti-Digoxigenin

from mouse IgG1κ (clone 1.71.256)

Synonym(s):

anti-digoxigenin, digoxigenin

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About This Item

UNSPSC Code:
12352203

biological source

mouse

Quality Level

100

conjugate

unconjugated

antibody form

purified immunoglobulin

antibody product type

primary antibodies

clone

clone 1.71.256, monoclonal

form

lyophilized

packaging

pkg of 100 μg

manufacturer/tradename

Roche

technique(s)

ELISA: 2-4 μg/mL
immunocytochemistry: 0.5-2 μg/mL
immunohistochemistry: 0.5-2 μg/mL
western blot: 0.5-2 μg/mL

isotype

IgG1κ

storage temp.

2-8°C

General description

Contents: Lyophilizate
Digoxigenin is a hapten, useful in labeling nucleic acids and in detection systems. Probes labeled with digoxigenin has greater sensitivity equivalent to that of radioactive probes, allows faster detection. It is less hazardous and has increased shelf life.

Specificity

The monoclonal antibody reacts with free and bound digoxigenin.

Application

Use Anti-Digoxigenin antibody for the detection of digoxigenin-labeled componds using:
  • ELISA
  • Immunohistocytochemistry
  • In situ hybridization
  • Western blot
  • Immunofluorescence staining
  • FISH (fluorescent in situ hybridization)

Biochem/physiol Actions

In the presence of Na+, Mg2+ and adenosine triphosphate (ATP), digoxigenin inhibits sodium pumps.

Features and Benefits

  • Specific to free and bound digoxigenin
  • The antibody is not stabilized with protein and therefore suitable as coating antibody in Sandwich-ELISAs and for labeling procedures. For example it can be conjugated with enzymes or fluorescence dyes for direct detection of digoxigenin-labeled samples.

Preparation Note

Working concentration: Working concentration of antibody depends on application and substrate. The following concentrations should be taken as a guideline:
  • ELISA: for coating: 2 to 4 μg/ml
  • Immunohistocytochemistry: 0.5 to 2 μg/ml
  • In situ hybridization: 0.2 to 0.4 μg/ml
  • Western blot: 0.5 to 2 μg/ml

Working solution: For coating applications
phosphate buffered saline, pH 7.4

Reconstitution

Add 1 ml double-distilled water to a final concentration of 100 μg/ml.

Storage and Stability

The lyophilized antibody is stable at +2 to +8°C. The reconstituted antibody solution is stable up to 6 months at +2 to +8°C. The solution can be stored in aliquots at -15 to -25°C. Avoid repeated freezing and thawing.

Analysis Note

There are no cross reactivities known.

Other Notes

For life science research only. Not for use in diagnostic procedures.

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Pictograms

Exclamation mark
GHS07

Signal Word

Warning

Hazard Statements

H317

Hazard Classifications

Skin Sens. 1

Storage Class Code

11 - Combustible Solids

WGK

WGK 3

Flash Point(F)

does not flash

Flash Point(C)

does not flash

Certificates of Analysis (COA)

Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.

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Nonradioactive labeling of probe with digoxigenin by polymerase chain reaction
<BIG>Lion T and Haas OA</BIG>
Analytical Chemistry, 188, 335-337 (1990)
Magda Rudzka et al.
The Plant cell, 34(6), 2404-2423 (2022-03-17)
Gene regulation ensures that the appropriate genes are expressed at the proper time. Nuclear retention of incompletely spliced or mature mRNAs is emerging as a novel, previously underappreciated layer of posttranscriptional regulation. Studies on this phenomenon indicated that it exerts
Carla Winterling et al.
RNA biology, 11(1), 66-75 (2014-01-21)
A growing body of evidence suggests the non-protein coding human genome is of vital importance for human cell function. Besides small RNAs, the diverse class of long non-coding RNAs (lncRNAs) recently came into focus. However, their relevance for infection, a
Affinity Labeling of a Sulfhydryl Group in the Cardiacglycoside Receptor Site of Na+/K+-ATPase by N-Hydroxysuccinimidyl Derivatives of Digoxigenin
<BIG>Antolovic R, et al.</BIG>
European Journal of Biochemistry, 227, 61-67 (1995)
Xingqi Chen et al.
BioTechniques, 56(3), 117-118 (2014-03-20)
Current techniques for analyzing chromatin structures are hampered by either poor resolution at the individual cell level or the need for a large number of cells to obtain higher resolution. This is a major problem as it hampers our understanding
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Articles

Digoxigenin (DIG) Labeling Methods

Digoxigenin (DIG) labeling methods and kits for DNA and RNA DIG probes, random primed DNA labeling, nick translation labeling, 5’ and 3’ oligonucleotide end-labeling.

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