SAE176-100UG

SAvPhire Monomeric Streptavidin Alpha Fluor 488

• UNSPSC Code: 12352203
• eCl@ss: 32160702
• NACRES: NA.46

Properties

• conjugate: horse radish peroxidase (HRP)
• Quality Level: 200
• clone: monoclonal
• species reactivity: all
• technique(s): flow cytometry: suitable; immunocytochemistry: suitable; western blot: suitable

Description

General description

Upon reconstitution in 100 μL of distilled water the concentration will be 1.0 mg/mL. Please refer to guidance on suggested starting dilutions and/or titers per application and sample type.

Specificity

SAvPhire Monomeric Streptavidin expressed in E. coli and conjugated with Alpha Fluor 488 that is suitable for immunodetection of biotinylated proteins.

Application

Epitope: Unknown

Quality

Evaluated by Immunocytochemistry in A431 cells.



Immunofluorescent analysis of A431 cells performed using a 1:1 mixture of biotinylated Anti-EGFR (1:1,000 dilution) and SAvPhire Monomeric Streptavidin Alpha Fluor 488 detected EGFR.

Physical properties

15.5 kDa for SAvPhire Monomeric Streptavidin (with an N-terminal His-tag and a C-terminal FLAG tag).

Physical form

SAvPhire Monomeric Streptavidin Alpha Fluor 488 lyophilized from PBS containing D-Mannitol and Sucrose without azide.

Storage and Stability

Stable for 1 year at +2°C to +8°C from date of receipt.

Other Notes

Predicted to react with all species.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Safety Information

• Storage Class Code: 11 - Combustible Solids
• WGK: WGK 3
• Flash Point(F): Not applicable
• Flash Point(C): Not applicable