MABT83
Anti-Aggrecan Antibody, clone 6B4
clone 6B4, from mouse
Synonym(s):
Aggrecan core protein, Cartilage-specific proteoglycan core protein, CSPCP, Chondroitin sulfate proteoglycan core protein 1, Chondroitin sulfate proteoglycan 1, Aggrecan core protein 2
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All Photos(2)
About This Item
UNSPSC Code:
12352203
eCl@ss:
32160702
NACRES:
NA.41
Recommended Products
biological source
mouse
Quality Level
antibody form
purified immunoglobulin
antibody product type
primary antibodies
clone
6B4, monoclonal
species reactivity
bovine
species reactivity (predicted by homology)
human (based on 100% sequence homology)
technique(s)
immunohistochemistry: suitable
western blot: suitable
isotype
IgG1κ
NCBI accession no.
UniProt accession no.
shipped in
wet ice
target post-translational modification
unmodified
Gene Information
human ... ACAN(176)
General description
Aggrecan (CSPG1, CSPCP, or CSPGCP) is a chondroitin-sulfate proteoglycan belonging to the family of letican proteins. Aggrecan is a large globar structure containing multiple domains including carbohydrate-recognition domains; complement binding protein domains; immunoglobulin domains; proteoglycan tandem repeats; and in some cases, EGF-like domains. It can be degraded by aggrecanase. The N-terminus of aggrecan binds ECM hyaluronan in the presence of the linking protein, and forms complex aggregates, whereas the C-terminus binds a number of other ECM proteins including tenascins, glycolipids, and fibulins. Aggrecan is a major component of cartilage tissue and it enables this tissue to withstand compression by maintaining the viso-elastic properties of cartilage. Aggrecan may also facilitate neural maturation and plasticity by contributing to the formation of perineuronal nets in the developing CNS.
Immunogen
Ovalbumin-conjugated linear peptide corresponding to human Aggrecan.
Application
Anti-Aggrecan Antibody, clone 6B4 is an antibody against Aggrecan for use in Western Blotting & IHC.
Immunofluorescent Analysis: A representative lot was used by an independent laboratory in human femoral chondrocytes (Williams, R., et al. (2010). PLoS One. 5(10):e13246.).
Immunohistochemistry Analysis: A representative lot was used by an independent laboratory in human chartilage tissue (Williams, R., et al. (2010). PLoS One. 5(10):e13246.).
Immunohistochemistry Analysis: A representative lot was used by an independent laboratory in human chartilage tissue (Williams, R., et al. (2010). PLoS One. 5(10):e13246.).
Research Category
Cell Structure
Cell Structure
Research Sub Category
ECM Proteins
ECM Proteins
Quality
Evaluated by Western Blot in A1D1 purified protein from bovine tissue lysate.
Western Blot Analysis: 1 µg/mL of this antibody detected Aggrecan in 10 µg of purified protein from bovine tissue lysate.
Western Blot Analysis: 1 µg/mL of this antibody detected Aggrecan in 10 µg of purified protein from bovine tissue lysate.
Target description
~60 kDa observed. Uniprot describes three isoforms at ~250 kDa, ~246 kDa, and ~239 kDa Aggrecan is degraded by aggrecanase into a ~60 kDa protein and other higher molecular weight products (Lemons, M. L. et al. (2001). J Neurosci. 21(13):4772-4781. Forsang, A. J. et al. (1995). Biochem J. 310(Pt 1):337-343.).
Physical form
Format: Purified
Protein G Purified
Purified mouse monoclonal IgG1κ in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.
Storage and Stability
Stable for 1 year at 2-8°C from date of receipt.
Analysis Note
Control
A1D1 purified protein from bovine tissue lysate
A1D1 purified protein from bovine tissue lysate
Other Notes
Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.
Disclaimer
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
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Storage Class Code
12 - Non Combustible Liquids
WGK
WGK 1
Flash Point(F)
Not applicable
Flash Point(C)
Not applicable
Certificates of Analysis (COA)
Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.
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Identification and clonal characterisation of a progenitor cell sub-population in normal human articular cartilage.
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Testing, 5, e13246-e13246 (2010)
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